Nuno S. Osório
University of Minho
Drug resistanceInternal medicineSingle-nucleotide polymorphismVirologyPhylogenetic treeEpitopeImmunologyLineage (genetic)Transmission (medicine)Mycobacterium tuberculosisPlasmodium falciparumMycobacterium tuberculosis complexTuberculosisSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Human immunodeficiency virus (HIV)Computer scienceNitric oxideGeneticsVirulenceMedicineBiologyImmune systemMicrobiologyPhylogeneticsCell biology
Publications 40
#1Eduarda Carvalho-Correia (University of Minho)H-Index: 2
#2Carla CalçadaH-Index: 2
Last. João Carlos Sousa (University of Minho)H-Index: 28
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Extensive transmission of SARS-CoV-2 during the COVID-19 pandemic allowed the generation of thousands of mutations within its genome. While several of these become rare, others largely increase in prevalence, potentially jeopardizing the sensitivity of PCR-based diagnostics. Taking advantage of SARS-CoV-2 genomic knowledge, we designed a one-step probe-based multiplex RT-qPCR (OmniSARS2) to simultaneously detect short fragments of the SARS-CoV-2 genome in ORF1ab, E gene and S gene. Comparative g...
#1George A. YendewaH-Index: 4
#2Sulaiman LakohH-Index: 6
Last. Nuno S. OsórioH-Index: 14
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Human immunodeficiency virus (HIV) drug resistance (HIVDR) is widespread in sub-Saharan Africa. Children and pregnant women are particularly vulnerable, and laboratory testing capacity remains limited. We, therefore, used a cross-sectional design and convenience sampling to characterize HIV subtypes and resistance-associated mutations (RAMs) in these groups in Sierra Leone. In total, 96 children (age 2–9 years, 100% ART-experienced), 47 adolescents (age 10–18 years, 100% ART-experienced), and 54...
#1Richard Thomson-Luque (University Hospital Heidelberg)H-Index: 7
#2Lasse Votborg-Novél (MPG: Max Planck Society)
Last. Boureima Guindo (University of the Sciences)H-Index: 2
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Following Plasmodium falciparum infection, individuals can remain asymptomatic, present with mild fever in uncomplicated malaria cases, or show one or more severe malaria symptoms. Several studies have investigated associations between parasite transcription and clinical severity, but no broad conclusions have yet been drawn. Here, we apply a series of bioinformatic approaches based on P. falciparum's tightly regulated transcriptional pattern during its ~48-hour intraerythrocytic developmental c...
The success of antiretroviral treatment (ART) is threatened by the emergence of drug resistance mutations (DRM). Since Brazil presents the largest number of people living with HIV (PLWH) in South America we aimed at understanding the dynamics of DRM in this country. We analyzed a total of 20,226 HIV-1 sequences collected from PLWH undergoing ART between 2008–2017. Results show a mild decline of DRM over the years but an increase of the K65R reverse transcriptase mutation from 2.23% to 12.11%. Th...
#1Nuno S. OsórioH-Index: 14
#2Maria Isabel VeigaH-Index: 14
#1Nuno S. Osório (University of Minho)H-Index: 14
#2Margarida Correia-Neves (University of Minho)H-Index: 34
30 CitationsSource
#1Ana Santos-Pereira (University of Minho)
#2Carlos Magalhães (University of Minho)H-Index: 4
Last. Nuno S. Osório (University of Minho)H-Index: 14
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The already enormous burden caused by Mycobacterium tuberculosis and Human Immunodeficiency Virus type 1 (HIV-1) alone is aggravated by co-infection. Despite obvious differences in the rate of evolution comparing these two human pathogens, genetic diversity plays an important role in the success of both. The extreme evolutionary dynamics of HIV-1 is in the basis of a robust capacity to evade immune responses, to generate drug-resistance and to diversify the population-level reservoir of M group ...
#1Maria Isabel Veiga (University of Minho)H-Index: 14
#2Nuno S. Osório (University of Minho)H-Index: 14
#2Nuno S. OsórioH-Index: 14
Last. Margarida SaraivaH-Index: 27
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