Adriano Aguzzi
University of Zurich
TransgeneGeneGenetically modified mousePathologyMolecular biologyVirologyAntibodyChemistryImmunologyScrapieNeurodegenerationPRNPDiseasePathogenesisBovine spongiform encephalopathyCentral nervous systemMedicineBiologyImmune systemCell biology
Publications 607
#1Daniel Heinzer (UZH: University of Zurich)H-Index: 2
#2Merve Avar (UZH: University of Zurich)H-Index: 2
Last. Adriano Aguzzi (UZH: University of Zurich)H-Index: 111
view all 15 authors...
The cellular prion protein PrPC is necessary for prion replication, and its reduction greatly increases life expectancy in animal models of prion infection. Hence the factors controlling the levels of PrPC may represent therapeutic targets against human prion diseases. Here we performed an arrayed whole-transcriptome RNA interference screen to identify modulators of PrPC expression. We cultured human U251-MG glioblas-toma cells in the presence of 64752 unique siRNAs targeting 21584 annotated hum...
#1Marco Losa (UZH: University of Zurich)H-Index: 2
#2Schuerch P (UZH: University of Zurich)
Last. Adriano Aguzzi (UZH: University of Zurich)H-Index: 111
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The apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) protein is a component of inflammasomes, inflammation-mediating complexes that include Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3 (NLRP3). ASC and NLRP3 components aggregate to form ASC specks, which can cross-seed A{beta} aggregation in Alzheimers disease (AD). Here we asked whether ASC is involved in additional protein misfolding diseases (PMDs). AA amyloi...
#1Marc Emmenegger (UZH: University of Zurich)H-Index: 6
#2De Cecco E (UZH: University of Zurich)
Last. Adriano Aguzzi (UZH: University of Zurich)H-Index: 111
view all 29 authors...
While the initial pathology of Parkinson9s disease and other α-synucleinopathies is often confined to circumscribed brain regions, it can spread and progressively affect adjacent and distant brain locales. This process may be controlled by cellular receptors of α-synuclein fibrils, one of which was proposed to be the LAG3 immune checkpoint molecule. Here, we analyzed the expression pattern of LAG3 in human and mouse brains. Using a variety of methods and model systems, we found no evidence for L...
#1Sebastian FiedlerH-Index: 7
Last. Tuomas P. J. Knowles (University of Cambridge)H-Index: 63
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The humoral immune response plays a key role in suppressing the pathogenesis of SARS-CoV-2. The molecular determinants underlying the neutralization of the virus remain, however, incompletely understood. Here, we show that the ability of antibodies to disrupt the binding of the viral spike protein to the angiotensin-converting enzyme 2 (ACE2) receptor on the cell, the key molecular event initiating SARS-CoV-2 entry into host cells, is controlled by the affinity of these antibodies to the viral a...
#1Luise Linsenmeier (UHH: University of Hamburg)H-Index: 6
#2Behnam MohammadiH-Index: 4
Last. Markus GlatzelH-Index: 52
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The cellular prion protein (PrPC) is a central player in neurodegenerative diseases caused by protein misfolding, such as prion diseases or Alzheimer's disease (AD). Expression levels of this GPI-anchored glycoprotein, especially at the neuronal cell surface, critically correlate with various pathomechanistic aspects underlying these diseases, such as templated misfolding (in prion diseases) and neurotoxicity and, hence, with disease progression and severity. In stark contrast to cell-associated...
#1Daniele FocosiH-Index: 23
#2Fabrizio Maggi (University of Insubria)H-Index: 32
Last. Francesco MenichettiH-Index: 39
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Abstract Background COVID19 convalescent plasma (CCP) is being extensively investigated as a treatment, with mixed results to date. Overall, there has been a generalized lack of appropriateness in prescriptions, which is termed patient-blood management in the field of transfusion medicine. Objectives We aimed at dissecting study design variables which could affect clinical outcome after CCP therapy. We focus here on variables such as pretransfusion antibody testing in recipients, dose adjustemen...
#1Daniel Kirschenbaum (UZH: University of Zurich)H-Index: 6
#2Lukas L. Imbach (UZH: University of Zurich)H-Index: 12
Last. Karl Frontzek (UZH: University of Zurich)H-Index: 10
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Coronavirus disease 19 (COVID-19), caused by infection with the severe acute respiratory syndrome coronavirus 2 (Sars-CoV-2), has become a worldwide pandemic (1). Symptoms of COVID-19 vary widely and range from asymptomatic disease to severe pneumonia and multiorgan failure (2). A severe disease course is more likely in older patients and patients with pre-existing respiratory and cardiovascular conditions (2). Patients with severe Sars-CoV-2 infection may present with ischaemic stroke (3, 4) or...
10 CitationsSource
#1Georg Meisl (University of Cambridge)H-Index: 27
#2Timothy D. Kurt (UCSD: University of California, San Diego)H-Index: 13
Last. Tuomas P. J. Knowles (University of Cambridge)H-Index: 63
view all 15 authors...
Prions consist of pathological aggregates of cellular prion protein and have the ability to replicate, causing neurodegenerative diseases, a phenomenon mirrored in many other diseases connected to protein aggregation, including Alzheimer’s and Parkinson’s diseases. However, despite their key importance in disease, the individual processes governing this formation of pathogenic aggregates, as well as their rates, have remained challenging to elucidate in vivo. Here we bring together a mathematica...
1 CitationsSource
#1Maximo Sanz-Hernandez (Imperial College London)H-Index: 4
#2Joseph D. Barritt (Imperial College London)H-Index: 4
Last. Alfonso De Simone (Imperial College London)H-Index: 33
view all 6 authors...
The misfolding and aggregation of the human prion protein (PrP) is associated with transmissible spongiform encephalopathies (TSEs). Intermediate conformations forming during the conversion of the cellular form of PrP into its pathological scrapie conformation are key drivers of the misfolding process. Here, we analyzed the properties of the C-terminal domain of the human PrP (huPrP) and its T183A variant, which is associated with familial forms of TSEs. We show that the mutation significantly e...
#1Christopher J. Love (MIT: Massachusetts Institute of Technology)
#1Christopher J. Love (MIT: Massachusetts Institute of Technology)H-Index: 3
Last. Adriano Aguzzi (UZH: University of Zurich)H-Index: 111
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Background and Purpose: The classic presentation of chronic (stage III) hemorrhagic stroke lesions is a fluid-filled cavity. In one of the most commonly used animal models of intracerebral hemorrha...