Annarita Favia
National Research Council
Protein arginine methyltransferase 5Cell migrationVascular endothelial growth factor AKinase insert domain receptorCalcium in biologyCalcium signalingNaringeninVascular endothelial growth factorNicotinic acid adenine dinucleotide phosphateAngiogenesisStem cellTube formationCancer researchBiochemistrySignal transductionProtein kinase BIntracellularBiologyMethyltransferaseCell biology
Publications 14
#1Annarita Favia (National Research Council)H-Index: 8
#2Luisa Salvatori (National Research Council)H-Index: 17
Last. Barbara Illi (National Research Council)H-Index: 21
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Protein Arginine (R) methylation is the most common post-translational methylation in mammalian cells. Protein Arginine Methyltransferases (PRMT) 1 and 5 dimethylate their substrates on R residues, asymmetrically and symmetrically, respectively. They are ubiquitously expressed and play fundamental roles in tumour malignancies, including glioblastoma multiforme (GBM) which presents largely deregulated Myc activity. Previously, we demonstrated that PRMT5 associates with Myc in GBM cells, modulatin...
8 CitationsSource
#1Pablo Suárez-Cortés (ISS: Istituto Superiore di Sanità)H-Index: 4
#2Guido Gambara (Sapienza University of Rome)H-Index: 8
Last. Antonio Filippini (Sapienza University of Rome)H-Index: 31
view all 6 authors...
Background Although malaria is a preventable and curable human disease, millions of people risk to be infected by the Plasmodium parasites and to develop this illness. Therefore, there is an urgent need to identify new anti-malarial drugs. Ca2+ signalling regulates different processes in the life cycle of Plasmodium falciparum, representing a suitable target for the development of new drugs.
4 CitationsSource
#1Irene Pafumi (Sapienza University of Rome)H-Index: 4
#2Margherita Festa (University of Milan)H-Index: 9
Last. Armando CarpanetoH-Index: 23
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Our research introduces the natural flavonoid naringenin as a novel inhibitor of an emerging class of intracellular channels, Two-Pore Channel 2 (TPC2), as shown by electrophysiological evidence in a heterologous system, i.e. Arabidopsis vacuoles lacking endogenous TPCs. In view of the control exerted by TPC2 on intracellular calcium signaling, we demonstrated that naringenin dampens intracellular calcium responses of human endothelial cells stimulated with VEGF, histamine or NAADP-AM, but not w...
30 CitationsSource
#1Pietro Laneve (IIT: Istituto Italiano di Tecnologia)H-Index: 18
#2Agnese Po (Sapienza University of Rome)H-Index: 19
Last. Elisa Caffarelli (National Research Council)H-Index: 21
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// Pietro Laneve 1 , Agnese Po 2 , Annarita Favia 3 , Ivano Legnini 4 , Vincenzo Alfano 1, 2 , Jessica Rea 4 , Valerio Di Carlo 4, 11 , Valeria Bevilacqua 4, 12 , Evelina Miele 1, 13 , Angela Mastronuzzi 5 , Andrea Carai 6 , Franco Locatelli 5, 7 , Irene Bozzoni 1, 3, 4, 8 , Elisabetta Ferretti 9, 10 , Elisa Caffarelli 1, 3 1 Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy 2 Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy ...
27 CitationsSource
Two Pore Channels (TPCs) are an emerging family of intracellular channels, expressed on acidic compartments, which mediate calcium signaling evoked by NAADP. In particular, we demonstrated that TPC2 isoform has a main role in angiogenesis (Favia et al. PNAS 2014 Nov 4;111(44):E4706-15). TPC2 inhibition is emerging as a key therapeutic step in a range of important pathological conditions including the progression and metastatic potential of cancer, Parkinson’s disease, and Ebola virus infection. ...
#1Annarita Favia (Sapienza University of Rome)H-Index: 8
#2Irene Pafumi (Sapienza University of Rome)H-Index: 4
Last. Antonio Filippini (Sapienza University of Rome)H-Index: 31
view all 13 authors...
A novel transduction pathway for the powerful angiogenic factor VEGF has been recently shown in endothelial cells to operate through NAADP-controlled intracellular release of Ca(2+). In the present report the possible involvement of NAADP-controlled Ca(2+) signaling in tumor vascularization, growth and metastatic dissemination was investigated in a murine model of VEGF-secreting melanoma. Mice implanted with B16 melanoma cells were treated with NAADP inhibitor Ned-19 every second day for 4 weeks...
18 CitationsSource
#1Maria Patrizia Mongiardi (National Research Council)H-Index: 11
#2Mauro Savino (National Research Council)H-Index: 3
Last. Barbara Illi (Casa Sollievo della Sofferenza)H-Index: 21
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Myc and Omomyc functionally associate with the Protein Arginine Methyltransferase 5 (PRMT5) in glioblastoma cells
24 CitationsSource
#1Irene Pafumi (Sapienza University of Rome)H-Index: 4
#2Annarita Favia (Sapienza University of Rome)H-Index: 8
Last. Antonio Filippini (Sapienza University of Rome)H-Index: 31
view all 7 authors...
Angiopoietins are vascular factors essential for blood vessel assembly and correct organization and maturation. This study describes a novel calcium-dependent machinery activated through Angiopoietin-1/2-Tie receptor system in HUVECs monolayer. Both cytokines were found to elicit intracellular calcium mobilization. Targeting intracellular Ca2+ signaling, antagonizing IP3 with 2-APB or cADPR with 8Br-cADPR, was found to modulate in vitro angiogenic responses to Angiopoietins in a specific way. 2-...
11 CitationsSource
We have recently identified a novel transduction pathway through which Vascular Endothelial Growth Factor (VEGF) controls experimentally induced neoangiogenesis, specifically involving endothelial VEGF receptor subtype 2 and the release of intracellular calcium from NAADP (Nicotinic Acid Adenosine Dinucleotide Phosphate) responsive acidic stores (1). We have now extended this research to an in vivo model of tumor angiogenesis and show that the pharmacologic NAADP inhibitor Ned-19 (2) impairs the...
#1Annarita Favia (Sapienza University of Rome)H-Index: 8
#2Marianna DesideriH-Index: 19
Last. Antonio Filippini (Sapienza University of Rome)H-Index: 31
view all 12 authors...
Vascular endothelial growth factor (VEGF) and its receptors VEGFR1/VEGFR2 play major roles in controlling angiogenesis, including vascularization of solid tumors. Here we describe a specific Ca2+ signaling pathway linked to the VEGFR2 receptor subtype, controlling the critical angiogenic responses of endothelial cells (ECs) to VEGF. Key steps of this pathway are the involvement of the potent Ca2+ mobilizing messenger, nicotinic acid adenine-dinucleotide phosphate (NAADP), and the specific engage...
95 CitationsSource
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