Bruno Péault
University of California, Los Angeles
CD34HaematopoiesisEndothelial stem cellPathologyEmbryonic stem cellCellular differentiationMolecular biologyStromal cellAdipose tissueMesenchymal stem cellProgenitor cellImmunologyAdult stem cellBone marrowPericyteStem cellPopulationMedicineBiologyCell biology
339Publications
74H-index
17.6kCitations
Publications 334
Newest
#1Isaac Shaw (Edin.: University of Edinburgh)H-Index: 4
#2Eoin O'Sullivan (Edin.: University of Edinburgh)H-Index: 16
Last. David A. Ferenbach (Edin.: University of Edinburgh)H-Index: 19
view all 9 authors...
The renal mesenchyme contains heterogeneous cells, including interstitial fibroblasts and pericytes, with key roles in wound healing. Although healing is impaired in aged kidneys, the effect of age and injury on the mesenchyme remains poorly understood. We characterized renal mesenchymal cell heterogeneity in young vs old animals and after ischemia-reperfusion-injury (IRI) using multiplex immunolabeling and single cell transcriptomics. Expression patterns of perivascular cell markers (α-SMA, CD1...
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#1Ginny Ching Yun Hsu (Johns Hopkins University)H-Index: 2
#2Masnsen Cherief (Johns Hopkins University)H-Index: 1
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 8 authors...
Intra-articular injection of mesenchymal stem cells has shown benefit for the treatment of osteoarthritis (OA).However, MSCs at the origin of these clinical results are heterogenous cell populations with limited cellular characterization. Here, two transgenic reporter mice were used to examine the differential effects of two precisely defined perivascular cell populations (Pdgfrα+ and Pdgfrβ+ cells) from white adipose tissue for alleviation of OA. Perivascular mesenchymal cells were isolated fro...
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#1Yoann Atlas (PSL Research University)H-Index: 1
#1Yoann Atlas (PSL Research University)
Last. Laurent Muller (PSL Research University)H-Index: 45
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ABSTRACT Blood perfusion of grafted tissue constructs is a hindrance to the success of stem cell-based therapies by limiting cell survival and tissue regeneration. Implantation of a pre-vascularized network engineered in vitro has thus emerged as a promising strategy for promoting blood supply deep into the construct, relying on inosculation with the host vasculature. We aimed to fabricate in vitro tissue constructs with mature microvascular networks, displaying perivascular recruitment and base...
1 CitationsSource
#1Carolyn A. Meyers (Johns Hopkins University)H-Index: 11
#2Chen-Chao Wang (UCLA: University of California, Los Angeles)H-Index: 5
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 9 authors...
Human pericytes are a perivascular cell population with mesenchymal stem cell properties, present in all vascularized tissues. Human pericytes have a distinct immunoprofile, which may be leveraged for purposes of cell purification. Adipose tissue is the most commonly used cell source for human pericyte derivation. Pericytes can be isolated by FACS (fluorescence-activated cell sorting), most commonly procured from liposuction aspirates. Pericytes have clonal multilineage differentiation potential...
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#1Ania Stefanska (Edin.: University of Edinburgh)H-Index: 3
#2Angela Briski (Edin.: University of Edinburgh)
Last. John J. Mullins (Edin.: University of Edinburgh)H-Index: 69
view all 6 authors...
Renal pericytes have a critical importance for angiogenesis and vascular remodeling, medullary blood flow regulation, and development of fibrosis. An emerging role for kidney pericytes is their ability to induce renin expression and synthesis. Here, we present methods for purification of human renal pericytes, their primary culture, and differentiation into renin-producing cells. Possible applications of these protocols include investigations into (1) renin cell recruitment mechanisms, (2) modul...
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#1Jiajia XuH-Index: 11
#2Yiyun WangH-Index: 5
Last. Aaron W. JamesH-Index: 45
view all 7 authors...
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#1Stefano Negri (University of Verona)H-Index: 4
#2Yiyun Wang (Johns Hopkins University)H-Index: 5
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 12 authors...
The vascular wall stores mesenchymal progenitor cells which are able to induce bone regeneration, via direct and paracrine mechanisms. Although much is known regarding perivascular cell regulation of osteoblasts, their regulation of osteoclasts, and by extension utility in states of high bone resorption, is not known. Here, human perivascular stem cells (PSCs) were used as a means to prevent autograft resorption in a gonadectomy-induced osteoporotic spine fusion model. Furthermore, the paracrine...
3 CitationsSource
#1Jiajia Xu (Johns Hopkins University)H-Index: 11
#2Yiyun Wang (Johns Hopkins University)H-Index: 5
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 16 authors...
Tissue resident mesenchymal stem/stromal cells (MSCs) occupy perivascular spaces. Profiling human adipose perivascular mesenchyme with antibody arrays identified 16 novel surface antigens, including endolysosomal protein CD107a. Surface CD107a expression segregates MSCs into functionally distinct subsets. In culture, CD107alow cells demonstrate high colony formation, osteoprogenitor cell frequency, and osteogenic potential. Conversely, CD107ahigh cells include almost exclusively adipocyte progen...
3 CitationsSource
#1Jiajia Xu (Johns Hopkins University)H-Index: 11
#2Yiyun Wang (Johns Hopkins University)H-Index: 5
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 16 authors...
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#1Yiyun Wang (Johns Hopkins University)H-Index: 5
#2Stefano Negri (Johns Hopkins University)H-Index: 4
Last. Aaron W. James (Johns Hopkins University)H-Index: 45
view all 8 authors...
Adipose-derived stem/stromal cells (ASCs) have been previously used for bone repair. However, significant cell heterogeneity exists within the ASC population which has the potential to result in un...
1 CitationsSource