Tran Tuoc
Ruhr University Bochum
Protein subunitCorticogenesisNeural stem cellEmbryonic stem cellGene expressionProgenitorChromatin remodelingNeuroscienceChemistryProgenitor cellNeocortexCortex (anatomy)Cerebral cortexCortex (botany)EpigeneticsNeurogenesisPAX6ChromatinTranscription factorBiologyCell biology
Publications 31
#1Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
#2Yuanbin Xie (HMU: Hainan Medical University)
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
view all 4 authors...
Dynamic modification of RNA affords proximal regulation of gene expression triggered by non-genomic or environmental changes. One such epitranscriptomic alteration in RNA metabolism is the installation of a methyl group on adenosine (m6A) known to be the most prevalent modified state of mRNA in the mammalian cell. The methylation machinery responsible for the dynamic deposition and recognition of m6A on mRNA is composed of subunits that play specific roles, including reading, writing and erasing...
#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
view all 5 authors...
Summary Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases. Traditional PPI assays are laborious, involving the generation of many truncated proteins. SPOT peptide assay allows high-throughput detection of domains essential for PPI by synthesizing several hundred peptides on a cellulose membrane. Here, we present a rapid SPOT peptide protocol for identify...
#1Pauline Antonie Ulmke (GAU: University of Göttingen)H-Index: 2
#2M. Sadman Sakib (German Center for Neurodegenerative Diseases)H-Index: 6
Last. Tran Tuoc (GAU: University of Göttingen)H-Index: 16
view all 15 authors...
Summary Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (−) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2+ IPCs versus TBR2− cells revealed differences in key factors involved in chromatid ...
#1Godwin Sokpor (GAU: University of Göttingen)H-Index: 8
#2Joachim Rosenbusch (GAU: University of Göttingen)H-Index: 11
Last. Jochen F. Staiger (GAU: University of Göttingen)H-Index: 48
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Abstract Cortical morphogenesis entails several neurobiological events, including proliferation and differentiation of progenitors, migration of neuroblasts, and neuronal maturation leading to functional neural circuitry. These neurodevelopmental processes are delicately regulated by many factors. Endosomal SNAREs have emerged as formidable modulators of neuronal growth, aside their well-known function in membrane/vesicular trafficking. However, our understanding of their influence on cortex for...
#1Kathleen Wung Bi-Lin (NUS: National University of Singapore)H-Index: 1
Last. Manvendra K. Singh (NUS: National University of Singapore)H-Index: 25
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The BAF complex plays an important role in the development of a wide range of tissues by modulating gene expression programs at the chromatin level. However, its role in neural crest development has remained unclear. To determine the role of the BAF complex, we deleted BAF155/BAF170, the core subunits required for the assembly, stability, and functions of the BAF complex in neural crest cells (NCCs). Neural crest-specific deletion of BAF155/BAF170 leads to embryonic lethality due to a wide range...
#1Cemil KerimogluH-Index: 15
Last. T. Tuoc
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Increase in the size of human neocortex, acquired in evolution, accounts for the unique cognitive capacity of humans. This expansion appears to reflect the evolutionarily-enhanced proliferative ability of basal progenitors (BPs) in mammalian cortex, which may have been acquired through epigenetic alterations in BPs. However, whether or how the epigenome in BPs differs across species is not known. Here, we report that histone H3 acetylation is a key epigenetic regulation in BP amplification and c...
#1M. Sadman Sakib (German Center for Neurodegenerative Diseases)H-Index: 6
#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
view all 5 authors...
Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized ...
2 CitationsSource
#1Pauline Antonie Ulmke (GAU: University of Göttingen)H-Index: 2
#2Yuanbin Xie (GAU: University of Göttingen)H-Index: 2
Last. Tran Tuoc (GAU: University of Göttingen)H-Index: 16
view all 12 authors...
Fine-tuned gene expression is crucial for neurodevelopment. The gene expression program is tightly controlled at different levels, including RNA decay. N6-methyladenosine (m6A) methylation-mediated degradation of RNA is essential for brain development. However, m6A methylation impacts not only RNA stability, but also other RNA metabolism processes. How RNA decay contribute to brain development is largely unknown. Here, we show that Exosc10, an RNA exonuclease subunit of the RNA exosome complex, ...
1 CitationsSource
#1Milka PringsheimH-Index: 5
#2Diana MitterH-Index: 13
Last. Knut Brockmann (GAU: University of Göttingen)H-Index: 46
view all 7 authors...
#1Yuanbin Xie (GAU: University of Göttingen)H-Index: 2
#2Ricardo Castro-Hernandez (GAU: University of Göttingen)H-Index: 2
Last. Tran Tuoc (GAU: University of Göttingen)H-Index: 16
view all 8 authors...
Chromatin remodeling factor BAF155 is an important regulator of many biological processes. As a core and scaffold subunit of the BAF (SWI/SNF-like) complex, BAF155 is capable of regulating the stability and function of the BAF complex. The spatiotemporal expression of BAF155 during embryogenesis is essential for various aspects of organogenesis, particularly in the brain development. However, our understanding of the mechanisms that regulate the expression and function of BAF155 is limited. Here...
12 CitationsSource