Hideaki Nagase
University of Oxford
Matrix metalloproteinaseInternal medicineEndocrinologyPathologyMolecular biologyChemistryExtracellular matrixAggrecanAggrecanaseOsteoarthritisADAMTSCollagenaseCartilageMatrix Metalloproteinase 3BiochemistryMetalloproteinaseStereochemistryMedicineBiologyCell biology
355Publications
103H-index
31.7kCitations
Publications 349
Newest
#1Ivan Kadurin (UCL: University College London)H-Index: 15
#2Shehrazade Dahimene (UCL: University College London)H-Index: 4
Last. Annette C. Dolphin (UCL: University College London)H-Index: 82
view all 8 authors...
The auxiliary 2{delta} subunits of voltage-gated calcium (CaV) channels are key to augmenting expression and function of CaV1 and CaV2 channels, and are also important drug targets in several therapeutic areas, including neuropathic pain. The 2{delta} proteins are translated as pre-proteins encoding both 2 and {delta}, and post-translationally proteolysed into 2 and {delta} subunits, which remain associated as a complex. In this study we have identified ADAM17 as a key protease involved in prote...
Source
#1C.Y. Yang (University of Oxford)H-Index: 1
#2Anastasios Chanalaris (University of Oxford)H-Index: 15
Last. Stephan A. Müller (TUM: Technische Universität München)H-Index: 7
view all 14 authors...
Abstract Objective The adamalysin metalloproteinase 15 (ADAM15) has been shown to protect against development of osteoarthritis in mice. Here, we have investigated factors that control ADAM15 levels in cartilage. Design Secretomes from wild-type and Adam15−/− chondrocytes were compared by label-free quantitative mass spectrometry. mRNA was isolated from murine knee joints, either with or without surgical induction of osteoarthritis on male C57BL/6 mice, and the expression of Adam15 and other rel...
1 CitationsSource
#1Anna Paola Carreca (ISMETT)H-Index: 7
#2Veronica M. Pravata (Dund.: University of Dundee)H-Index: 4
Last. Simone D. Scilabra (ISMETT)H-Index: 8
view all 8 authors...
Matrix metalloproteinases (MMPs) and the related families of disintegrin metalloproteinases (ADAMs) and ADAMs with thrombospondin repeats (ADAMTSs) play a crucial role in extracellular matrix (ECM) turnover and shedding of cell-surface molecules. The proteolytic activity of metalloproteinases is post-translationally regulated by their endogenous inhibitors, known as tissue inhibitors of metalloproteinases (TIMPs). Several MMPs, ADAMTSs and TIMPs have been reported to be endocytosed by the low-de...
5 CitationsSource
#1Anna Paola Carreca (ISMETT)H-Index: 7
#2Veronica M. Pravata (Dund.: University of Dundee)H-Index: 4
Last. Simone D. Scilabra (ISMETT)H-Index: 8
view all 6 authors...
Matrix metalloproteinases (MMPs) and the related families of disintegrin metalloproteinases (ADAMs) and ADAMs with thrombospondin repeats (ADAMTSs) play a crucial role in the turnover of extracellular matrix (ECM) components and shedding of cell-surface molecules. The proteolytic activity of metalloproteinases is post-translationally regulated by their endogenous inhibitors, known as tissue inhibitors of metalloproteinases (TIMPs). Several MMPs, ADAMTSs and TIMPs have been reported to be endocyt...
Source
#1Rebar N. Mohammed (University of Sulaymaniyah)H-Index: 7
#2Sophie C. Wehenkel (Cardiff University)H-Index: 4
Last. Ann Ager (Cardiff University)H-Index: 36
view all 22 authors...
L-selectin on T-cells is best known as an adhesion molecule that supports recruitment of blood-borne naive and central memory cells into lymph nodes. Proteolytic shedding of the ectodomain is thought to redirect activated T-cells from lymph nodes to sites of infection. However, we have shown that activated T-cells re-express L-selectin before lymph node egress and use L-selectin to locate to virus-infected tissues. Therefore, we considered other roles for L-selectin proteolysis during T cell act...
6 CitationsSource
#1Kristin Schubert (Arthritis Research UK)H-Index: 10
#2Laura E. Collins (Arthritis Research UK)H-Index: 2
Last. Linda Troeberg (Arthritis Research UK)H-Index: 28
view all 5 authors...
The metalloproteinase ADAM17 plays a pivotal role in initiating inflammation by releasing TNF from its precursor. Prolonged TNF release causes many chronic inflammatory diseases, indicating that tight regulation of ADAM17 activity is essential for resolution of inflammation. In this study, we report that the endogenous ADAM17 inhibitor TIMP-3 inhibits ADAM17 activity only when it is bound to the cell surface and that cell surface levels of TIMP-3 in endotoxin-activated human macrophages are dyna...
9 CitationsSource
#1Aroa Duro-CastanoH-Index: 10
#2Ngee Han Lim (University of Oxford)H-Index: 16
Last. Hideaki Nagase (University of Oxford)H-Index: 103
view all 13 authors...
Imaging early molecular changes in osteoarthritic (OA) joints is instrumental for the development of disease‐modifying drugs. To this end, a fluorescent resonance energy transfer‐based peptide probe that is cleavable by matrix metalloproteinase 13 (MMP‐13) has been developed. This protease degrades type II collagen, a major matrix component of cartilage. The probe exhibits high catalytic efficiency (kcat/KM = 6.5 × 105m−1 s−1) and high selectivity for MMP‐13 over a set of nine MMPs. To achieve o...
19 CitationsSource
#1Arya Mekkat (Tufts University)H-Index: 3
#2Erik Poppleton (Tufts University)H-Index: 3
Last. Yu-Shan Lin (Tufts University)H-Index: 23
view all 8 authors...
Abstract Cleavage of collagen by collagenases such as matrix metalloproteinase 1 (MMP-1) is a key step in development, tissue remodeling, and tumor proliferation. The abundant heterotrimeric type I collagen composed of two α1(I) chains and one α2(I) chain is efficiently cleaved by MMP-1 at a unique site in the triple helix, a process which may be initiated by local unfolding within the peptide chains. Atypical homotrimers of the α1(I) chain, found in embryonic and cancer tissues, are very resist...
9 CitationsSource
#1Kazuhiro Yamamoto (University of Liverpool)H-Index: 17
#2C. Scavenius (AU: Aarhus University)H-Index: 1
Last. Hideaki Nagase (University of Oxford)H-Index: 103
view all 10 authors...
2 CitationsSource
#1Jacob Lund (Novo Nordisk)H-Index: 4
#2Anne Mette Elimar Bitsch (Novo Nordisk)H-Index: 1
Last. Helle Heibroch Petersen (Novo Nordisk)H-Index: 8
view all 9 authors...
Decysin-1 (ADAMDEC1) is an orphan ADAM-like metalloprotease with unknown biological function and a short domain structure. ADAMDEC1 mRNA has previously been demonstrated primarily in macrophages and mature dendritic cells. Here, we generated monoclonal antibodies (mAbs) against the mature ADAMDEC1 protein, as well as mAbs specific for the ADAMDEC1 pro-form, enabling further investigations of the metalloprotease. The generated mAbs bind ADAMDEC1 with varying affinity and represent at least six di...
3 CitationsSource