Robyn C. Kiser
Battelle Memorial Institute
GeneMatrix metalloproteinasePathologyGene expressionMolecular biologyGene expression profilingChemistryMicroarrayApoptosisImmunologyIn vivoGelatinase AGelatinaseInflammationWound healingSulfur mustardEdemaCell cycleToxicityTranscription (biology)BiochemistrySignal transductionMedicineMicroarray analysis techniquesBiologyDNA microarrayPharmacology
15Publications
9H-index
330Citations
Publications 15
Newest
#1Jennifer A. Price (Battelle Memorial Institute)H-Index: 8
#2James V. Rogers (Battelle Memorial Institute)H-Index: 16
Last. John S. GrahamH-Index: 18
view all 8 authors...
Bromine is an industrial chemical that causes severe cutaneous burns. When selecting or developing effective treatments for bromine burns, it is important to understand the molecular mechanisms of tissue damage and wound healing. This study investigated the effect of cutaneous bromine vapor exposure on gene expression using a weanling swine burn model by microarray analysis. Ventral abdominal sites were exposed to a mean calculated bromine vapor concentration of 0.51 g/L for 7 or 17 min. At 6 h,...
2 CitationsSource
#1James V. Rogers (Battelle Memorial Institute)H-Index: 16
#2Jennifer A. Price (Battelle Memorial Institute)H-Index: 8
Last. John S. GrahamH-Index: 18
view all 7 authors...
Bromine is an industrial chemical that can cause severe cutaneous burns. This study was a preliminary investigation into the effect of cutaneous exposure to bromine vapor using a weanling swine burn model and microarray analysis. Ventral abdominal sites were exposed to a mean calculated bromine vapor concentration of 0.69 g L−1 for 10 or 20 min. At 48 h postexposure, total RNA from skin samples was isolated, processed, and hybridized to Affymetrix GeneChip Porcine Genome Arrays. Expression analy...
12 CitationsSource
#1Jennifer A. Price (Battelle Memorial Institute)H-Index: 8
#2James V. Rogers (Battelle Memorial Institute)H-Index: 16
Last. John S. GrahamH-Index: 18
view all 7 authors...
Abstract Bromine is an industrial chemical that is irritating to the skin and causes cutaneous burns. An important factor in selecting or developing an effective treatment is to understand the underlying molecular mechanisms of tissue damage and wound healing. This study used a weanling swine burn model and microarray analysis to evaluate the effect of exposure length and sampling times on the transcriptional changes in response to cutaneous bromine injury. Ventral abdominal sites ( N = 4/treatm...
10 CitationsSource
#1Michael P. Shakarjian (RU: Rutgers University)H-Index: 12
#2Pinaki Bhatt (RU: Rutgers University)H-Index: 3
Last. Donald R. Gerecke (RU: Rutgers University)H-Index: 18
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Matrix metalloproteinases (MMPs), a class of enzymes responsible for the degradation of extracellular matrix proteins, play important roles in inflammatory and immune responses. In skin, MMP-2 (gelatinase A) and MMP- 9 (gelatinase B) are normally inactive but can be expressed during tissue injury. Both degrade collagen IV and other critical components of the basement membrane zone that separates the epidermis from the dermis. The expression of MMP-2 and -9 was studied in sulfur mustard (SM)-expo...
65 CitationsSource
#1James F. Dillman (United States Army Medical Research Institute of Chemical Defense)H-Index: 16
#1James F. Dillman (United States Army Medical Research Institute of Chemical Defense)H-Index: 2
Last. Carol L. K. SabourinH-Index: 24
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Bis-(2-chloroethyl) sulfide (sulfur mustard; SM) is a potent alkylating agent. Three treatment compounds have been shown to limit SM damage in the mouse ear vesicant model: dimercaprol, octyl homovanillamide, and indomethacin. Microarrays were used to determine gene expression profiles of biopsies taken from mouse ears after exposure to SM in the presence or absence of treatment compounds. Mouse ears were topically exposed to SM alone or were pretreated for 15 min with a treatment compound and t...
29 CitationsSource
#1James V. Rogers (Battelle Memorial Institute)H-Index: 16
#2Young W. Choi (Battelle Memorial Institute)H-Index: 13
Last. Carol L. K. Sabourin (Battelle Memorial Institute)H-Index: 24
view all 7 authors...
The chemical warfare agent sulfur mustard [bis-(2-chloroethyl)-sulfide; SM] produces a delayed inflammatory response followed by blister formation in skin of exposed individuals. Studies are underway evaluating the efficacy of pharmacological compounds to protect against SM skin injury. Microarray analysis provides the opportunity to identify multiple transcriptional biomarkers associated with SM exposure. This study examined SM-induced changes in gene expression in skin from mice cutaneously ex...
32 CitationsSource
#1Carol L. K. Sabourin (Battelle Memorial Institute)H-Index: 24
#2James V. Rogers (Battelle Memorial Institute)H-Index: 16
Last. John J. SchlagerH-Index: 41
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The chemical warfare agent sulfur mustard (SM) produces blister formation with a severe inflammatory reaction in skin of exposed individuals. The development of efficacious countermeasures against SM vesication requires an understanding of the cellular and molecular mechanism of SM-induced tissue injury. This study examined SM-induced alterations in gene expression using Atlas Mouse 5K DNA microarrays (5002 genes) to identify transcriptional events associated with SM skin injury. Mice (N = 3) we...
22 CitationsSource
#1Carol L. K. Sabourin (Battelle Memorial Institute)H-Index: 24
#2James V. Rogers (Battelle Memorial Institute)H-Index: 16
Last. Robert P. Casillas (Battelle Memorial Institute)H-Index: 20
view all 9 authors...
Sulfur mustard [bis(2‐chloroethyl)sulfide, SM] is a chemical warfare agent that penetrates the skin rapidly and causes extensive blistering. Using the mouse ear vesicant model (MEVM), we evaluated the effect of topically applied anti‐inflammatory agents (octyl homovanillamide and heptyl isovanillamide) on ear edema formation and gene expression following SM exposure. Relative ear weight and real‐time reverse transcriptase polymerase chain reaction of GM‐CSF, IL‐1β, and IL‐6 were used to evaluate...
9 CitationsSource
#1Donald R. GereckeH-Index: 18
#2Pinaki BhattH-Index: 3
Last. Michael P. ShakarjianH-Index: 12
view all 9 authors...
Epidermolysis bullosa (EB), a genetic skin disease characterized by skin blistering, may be caused by mutations in any of a variety of genes that encode proteins required for maintaining the structural integrity of skin. Three of these genes, laminin-α3, laminin-β3, and laminin-γ2 produce separate polypeptides that together form the basement membrane protein, laminin 5. Disruption of laminin 5 protein causes skin blisters that may be further enhanced by the actions of matrix metalloproteinases (...
#1Michael C. Babin (Battelle Memorial Institute)H-Index: 12
#2Karen M. RickettsH-Index: 6
Last. Robert P. Casillas (Battelle Memorial Institute)H-Index: 20
view all 11 authors...
The mouse ear vesicant model (MEVM) is a screening tool used to identify protective compounds against acute sulfur mustard (SM)‐induced skin injury. It provides endpoints of edema and histopathology 24 h following a topical SM exposure to assess protection against inflammation and tissue damage. To further evaluate successful compounds, the MEVM was modified for use as a 7‐day model. Dose response studies were conducted with SM to select an optimal challenge dose for the new model. Due to severi...
5 CitationsSource