Robert F. Diegelmann
Virginia Commonwealth University
Internal medicineEndocrinologySurgeryPathologyMolecular biologyAndrologyFetusChemistryExtracellular matrixIn vitroImmunologyInflammationHydroxyprolineProlineWound healingFibroblastBiochemistryMedicineBiologyCell biology
Publications 158
#1Stephanie Goldberg (VCU: Virginia Commonwealth University)H-Index: 10
#2Robert F. Diegelmann (VCU: Virginia Commonwealth University)H-Index: 1
Last. Robert F. Diegelmann (VCU: Virginia Commonwealth University)H-Index: 63
view all 2 authors...
Chronic wounds present a unique therapeutic challenge to heal. Chronic wounds are colonized with bacteria and the presence of a biofilm that further inhibits the normal wound healing processes, and are locked into a very damaging proinflammatory response. The treatment of chronic wounds requires a coordinated approach, including debridement of devitalized tissue, minimizing bacteria and biofilm, control of inflammation, and the use of specialized dressings to address the specific aspects of the ...
5 CitationsSource
#1H. Patrick MacKnight (USF: University of South Florida)H-Index: 2
#2Daniel J. Stephenson (USF: University of South Florida)H-Index: 3
Last. Charles E. ChalfantH-Index: 35
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The sphingolipid ceramide 1-phosphate (C1P) directly binds to and activates group IVA cytosolic phospholipase A2 (cPLA2α) to stimulate the production of eicosanoids. Because eicosanoids are important in wound healing, we examined the repair of skin wounds in knockout (KO) mice lacking cPLA2α and in knock-in (KI) mice in which endogenous cPLA2α was replaced with a mutant form having an ablated C1P interaction site. Wound closure rate was not affected in the KO or KI mice, but wound maturation was...
4 CitationsSource
#1Xi GaoH-Index: 3
#1Xi Gao (VCU: Virginia Commonwealth University)H-Index: 2
Last. Robert F. Diegelmann (VCU: Virginia Commonwealth University)H-Index: 63
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OBJECTIVE: The healing of wounds is critical in protecting the human body against environmental factors. The mechanisms involving protein expression during this complex physiological process have not been fully elucidated. APPROACH: Here, we use reverse-phase protein microarrays (RPPA) involving 94 phosphoproteins to study tissue samples from tubes implanted in healing dermal wounds in seven human subjects tracked over two weeks. We compare the proteomic profiles to proteomes of controls obtaine...
4 CitationsSource
#1Stephanie R. Goldberg (MCV: VCU Medical Center)H-Index: 9
#2Robert F. Diegelmann (MCV: VCU Medical Center)H-Index: 63
Normally the process of wound healing is a highly complex but well-orchestrated sequence of cellular and biochemical events that results in the reestablishment of tissue integrity and function. As the metabolic activity at the site of tissue injury intensifies, there is a high demand for nutrients and oxygen to support the repair processes. Oxygen is required for the proper synthesis and assembly of collagen and other extracellular matrix elements. In contrast, in pathologic states such as diabe...
1 CitationsSource
#1Racheal L. Cooper (MCV: VCU Medical Center)H-Index: 1
#2Rebecca Segal (MCV: VCU Medical Center)H-Index: 12
Last. Angela Reynolds (MCV: VCU Medical Center)H-Index: 9
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Abstract The normal wound healing response is characterized by a progression from clot formation, to an inflammatory phase, to a repair phase, and finally, to remodeling. In many chronic wounds there is an extended inflammatory phase that stops this progression. In order to understand the inflammatory phase in more detail, we developed an ordinary differential equation model that accounts for two systemic mediators that are known to modulate this phase, estrogen (a protective hormone during woun...
8 CitationsSource
#1Dayanjan S. Wijesinghe (VCU: Virginia Commonwealth University)H-Index: 21
#2Matthew Brentnall (UM: University of Miami)H-Index: 4
Last. Charles E. Chalfant (VCU: Virginia Commonwealth University)H-Index: 8
view all 7 authors...
In these studies, the role of ceramide-1-phosphate (C1P) in the wound-healing process was investigated. Specifically, fibroblasts isolated from mice with the known anabolic enzyme for C1P, ceramide kinase (CERK), ablated (CERK−/− mice) and their wild-type littermates (CERK+/+) were subjected to in vitro wound-healing assays. Simulation of mechanical trauma of a wound by scratching a monolayer of fibroblasts from CERK+/+ mice demonstrated steadily increasing levels of arachidonic acid in a time-d...
43 CitationsSource
#1Tomasz ArodzH-Index: 6
#2Danail Bonchev (VCU: Virginia Commonwealth University)H-Index: 17
Last. Robert F. Diegelmann (VCU: Virginia Commonwealth University)H-Index: 63
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Objective: The wound healing process is well-understood on the cellular and tissue level; however, its complex molecular mechanisms are not yet uncovered in their entirety. Viewing wounds as perturbed molecular networks provides the tools for analyzing and optimizing the healing process. It helps to answer specific questions that lead to better understanding of the complexity of the process. What are the molecular pathways involved in wound healing? How do these pathways interact with each other...
12 CitationsSource
#1Thomas L. Shaak (VCU: Virginia Commonwealth University)H-Index: 1
#2Dayanjan S. Wijesinghe (VCU: Virginia Commonwealth University)H-Index: 21
Last. Roger M. Loria (VCU: Virginia Commonwealth University)H-Index: 21
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DHEA, 17α-AED, 17β-AED, and 17β-AET exhibit strong biological activity that has been attributed to androgenic, estrogenic, or antiglucocorticoid activity in vivo and in vitro. This study compared DHEA, 17α-AED, 17β-AED, and 17β-AET for their ability to activate the human AR, ER, and GR and determine the relative androgenicity, estrogenicity, and glucocorticoid activity. The results show that, at the receptor level, these androstene hormones are weak AR and even weaker ER activators. Direct andro...
4 CitationsSource
#1Varun Oswal (VCU: Virginia Commonwealth University)H-Index: 1
#2Ashwin Belle (VCU: Virginia Commonwealth University)H-Index: 11
Last. Kayvan Najarian (VCU: Virginia Commonwealth University)H-Index: 25
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The segmentation and quantification of cell nuclei are two very significant tasks in the analysis of histological images. Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. In this paper, an automated entropy-based thresholding system for segmentation and quantification of cell nuclei from histological...
15 CitationsSource
#1Gayle M. Gordillo (OSU: Ohio State University)H-Index: 28
Last. Susan W. VolkH-Index: 24
view all 14 authors...
Abstract Significance: A review of therapeutic effects in preclinical and clinical studies suggests that concordance between large animal (pig=78%), small laboratory animal (53%) and in vitro (57%) results with those observed in humans is only partial. Pig models of wound healing provide major advantages over other animal models. Since the vast majority of wound-healing research is done in rodents and in vitro, the low concordance rate is a significant impediment to research that will have any c...
31 CitationsSource