Javier Eduardo García-Castañeda
National University of Colombia
AnalyteCytotoxicityConjugated systemDrug resistanceCancerMultiple drug resistanceGlycopeptideNuclear chemistryElectrospinningCorpus albicansMonolithMolecular biologyCytotoxic T cellPeptide sequenceChemistryApoptosisCombinatorial chemistryMinimum bactericidal concentrationMDA-MB-468LysineSolid-phase synthesisThesaurus (information retrieval)ArgininePseudomonas aeruginosaSequence (biology)Caffeic acidLactoferrinPullulanPeptideStaphylococcus aureusAntifungalPeptide synthesisAlanineEnterococcus faecalisHemolysisOxolinic acidContext (language use)Antibacterial activityMaleimideFerulic acidAlanine scanningFluconazoleLauric acidLactoferricinBuforin IIBactericidal effectChemical bindingAntibacterial peptideBovine lactoferricinColi strainChimera (genetics)Programmed cell deathMoleculePalindromeAntimicrobialCancer researchAmino acidBreast cancerAntimicrobial peptidesEscherichia coliFlow cytometryBiochemistryStrain (chemistry)Click chemistryComputational biologyStereochemistryCell cultureBiologyMicrobiologyNanofiberPolyclonal antibodies
14Publications
3H-index
50Citations
Publications 15
Newest
#1Nicolás Mateo González-López (National University of Colombia)
#2Kevin Andrey Huertas‐Ortiz (National University of Colombia)
Last. Zuly Jenny Rivera-Monroy (National University of Colombia)H-Index: 7
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Mycotoxins are secondary metabolites that are known to be toxic to humans and animals. On the other hand, some mycotoxins and their analogues possess antioxidant as well as antitumor properties, which could be relevant in the fields of pharmaceutical analysis and food research. Omics techniques are a group of analytical tools applied in the biological sciences in order to study genes (genomics), mRNA (transcriptomics), proteins (proteomics), and metabolites (metabolomics). Omics have become a vi...
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Monomeric, dimeric, palindromic, and tetrameric peptides derived from bovine lactoferricin (LfcinB) containing the RRWQWR or the KKWQWK sequence were synthesized via SPPS- Fmoc/tBu strategy, and Lys-peptides showed higher synthesis efficiency. The antibacterial activity of the peptides against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC reference strains and a multidrug resistant clinical isolate was evaluated. Lys peptides exhibited similar antibacterial activity to...
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Antimicrobial resistance is a public health problem, requiring the development of new therapeutic agents. Antimicrobial peptides derived from Bovine Lactoferricin (LfcinB) have exhibited antibacterial and antifungal effects. Lipopeptides and peptides containing non-natural amino acids, derived from LfcinB (21-25) Pal: RWQWRWQWR, were synthesized by SPPS-Fmoc/tBu and characterized by RP-HPLC and MALDI-TOF mass spectrometry. The antimicrobial activity was tested against reference strains E. coli A...
#1Verónica Rodríguez (National University of Colombia)H-Index: 2
#2Julieth Tatiana Román (National University of Colombia)H-Index: 2
Last. Javier Eduardo García-Castañeda (National University of Colombia)H-Index: 3
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#1Hector Manuel Pineda-Castañeda (National University of Colombia)H-Index: 1
#2Kevin Andrey Huertas‐Ortiz (National University of Colombia)
Last. Zuly Jenny Rivera-Monroy (National University of Colombia)H-Index: 7
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Chimeric peptides containing short sequences derived from bovine Lactoferricin (LfcinB) and Buforin II (BFII) were synthetized using solid-phase peptide synthesis (SPPS) and characterized via reverse-phase liquid chromatography and mass spectrometry. The chimeras were obtained with high purity, demonstrating their synthetic viability. The chimeras' antibacterial activity against Gram-positive and Gram-negative strains was evaluated. Our results showed that all the chimeras exhibited greater anti...
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Metabolites have great potential for the design of biomarkers, since their presence or absence provides valuable information about a biological system. In this context, polyclonal antibodies are important metabolites for diagnostic procedures, but in some pathologies, it has been found that these metabolites are present at low concentrations, so it could be difficult to detect them. In this investigation, an organic monolithic material of poly(GMA-co-EDMA) was functionalized with a peptide via M...
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#1Andrea Barragán-Cárdenas (National University of Colombia)H-Index: 1
#2Diego Sebastián Insuasty-Cepeda (National University of Colombia)H-Index: 1
Last. Javier Eduardo García-Castañeda (National University of Colombia)H-Index: 3
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1 CitationsSource
#1Natalia Ardila-Chantré (National University of Colombia)H-Index: 1
Last. Javier Eduardo García-Castañeda (National University of Colombia)H-Index: 3
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Short peptides derived from buforin and lactoferricin B were conjugated with other antimicrobial molecules of different chemical natures. The sequences RLLR, RLLRLLR, RWQWRWQWR, and RRWQWR were conjugated at their N-terminal end with non-peptidic molecules such as 6-aminohexanoic acid, ferrocene, caffeic acid, ferulic acid, and oxolinic acid. Peptide conjugates and unmodified peptides were synthesized by means of solid-phase peptide synthesis using the Fmoc/tBu strategy (SPPS-Fmoc/tBu), purified...
2 CitationsSource
#1Yerly Vargas-Casanova (Pontifical Xavierian University)H-Index: 1
#2Jean Carlos Villamil Poveda (Pontifical Xavierian University)H-Index: 1
Last. Claudia Marcela Parra Giraldo (Pontifical Xavierian University)H-Index: 2
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4 CitationsSource
#1Diego Sebastián Insuasty-Cepeda (National University of Colombia)H-Index: 1
#2Andrea Barragán-Cárdenas (National University of Colombia)H-Index: 1
Last. Zuly Jenny Rivera-Monroy (National University of Colombia)H-Index: 7
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The effect on the cytotoxicity against breast cancer cell lines of the substitution of 26Met residue in the sequence of the Bovine Lactoferricin-derived dimeric peptide LfcinB (20-30)2: (20RRWQWRMKKLG30)2-K-Ahx with amino acids of different polarity was evaluated. The process of the synthesis of the LfcinB (20-30)2 analog peptides was similar to the original peptide. The cytotoxic assays showed that some analog peptides exhibited a significant cytotoxic effect against breast cancer cell lines HT...
2 CitationsSource