Kai Kehe
Ludwig Maximilian University of Munich
CytotoxicityHaCaTNuclear chemistryDNA damageMolecular biologyChemistryApoptosisMaterials scienceIn vitroImmunologyIn vivoNerve agentSulfur mustardToxicityNecrosisBiochemistryMedicineCell cultureBiologyCell biologyPharmacology
81Publications
30H-index
2,876Citations
Publications 78
Newest
#1Noemi Colozza (University of Rome Tor Vergata)H-Index: 7
#2Kai KeheH-Index: 30
Last. Fabiana Arduini (University of Rome Tor Vergata)H-Index: 47
view all 6 authors...
Herein, we report a novel paper-based electrochemical sensor for on-site detection of sulphur mustards. This sensor was conceived combining office paper-based electrochemical sensor with choline oxidase enzyme to deliver a sustainable sensing tool. The mustard agent detection relies on the evaluation of inhibition degree of choline oxidase, which is reversibly inhibited by sulphur mustards, by measuring the enzymatic by-product H2O2 in chronoamperometric mode. A nanocomposite constituted of Prus...
8 CitationsSource
#1Noemi Colozza (University of Rome Tor Vergata)H-Index: 7
#2Vincenzo Mazzaracchio (University of Rome Tor Vergata)H-Index: 8
Last. Fabiana Arduini (University of Rome Tor Vergata)H-Index: 47
view all 9 authors...
Abstract Herein, a novel configuration for the detection of a chemical warfare agent, namely sulfur mustard (bis(2-chloroethyl)sulphide) was designed to develop a biosensor that can play a relevant role in the monitoring of such a chemical threat in risk and war areas. In detail, a tailor-made heterogeneous oligonucleotide-antibody biosensor was realised, based on the formation of a sulfur mustard-oligonucleotide adduct on the surface of a screen-printed electrode. Thus, the immunoassay was carr...
1 CitationsSource
#1Simone RothmillerH-Index: 6
#2Frank BalszuweitH-Index: 15
Last. Annette Schmidt (Bundeswehr University Munich)
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Abstract In vitro cell culture experiments are highly important techniques to accelerate drug discovery, conduct safety testing and reduce the need for animal studies. Therefore, automatization may help to enhance the technical precision, reduce external (including operator’s) influence on the data and thus improve reliability. Prior to application in scientific studies, validation of automated systems is absolutely necessary. In this study we present the validation of two combined automated pip...
1 CitationsSource
#1Dirk Steinritz (LMU: Ludwig Maximilian University of Munich)H-Index: 22
#2Simon LangH-Index: 3
Last. Kai Kehe (LMU: Ludwig Maximilian University of Munich)H-Index: 30
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Abstract Vesicants cause a multitude of cutaneous reactions like erythema, blisters and ulcerations. After exposure to sulfur mustard (SM) and related compounds, patients present dermal symptoms typically known for chemicals categorized as skin sensitizer (e.g. hypersensitivity and flare-up phenomena). However, although some case reports led to the assumption that SM and other alkylating compounds represent sensitizers, a comprehensive investigation of SM-triggered immunological responses has no...
4 CitationsSource
#1Simon WagnerH-Index: 1
#2Simon LangH-Index: 3
Last. Kai Kehe (LMU: Ludwig Maximilian University of Munich)H-Index: 30
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Abstract Sulfur mustard (SM) is a highly toxic chemical warfare agent, which produces blisters after skin contact. Treatment of SM-induced adverse health effects, such as cutaneous blistering, ulceration, and inflammation remains a challenging task. Antidotes or specific therapeutic measures are lacking. Some drugs (e.g. cyclooxygenase (COX) inhibitors) exhibited beneficial effects after SM poisoning in vivo. However, in vitro studies that evaluate and compare the potency of COX inhibitors are m...
6 CitationsSource
#1Noemi Colozza (University of Rome Tor Vergata)H-Index: 7
#2Kai Kehe (LMU: Ludwig Maximilian University of Munich)H-Index: 30
Last. Fabiana Arduini (University of Rome Tor Vergata)H-Index: 47
view all 8 authors...
Abstract The synthesis and employment of volatile toxic compounds as chemical weapons with a large-scale destructive power has introduced a new insidious threat over the last century. In this framework, the development of wearable sensing tools represents a critical point within the security field, in order to provide early alarm systems. Herein, a novel wearable electrochemical biosensor was developed for the rapid and on-site detection of mustard agents. Since a chemical attack is typically ca...
46 CitationsSource
#1Georg MenacherH-Index: 4
#2Frank BalszuweitH-Index: 15
Last. Tanja Popp (LMU: Ludwig Maximilian University of Munich)H-Index: 15
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Abstract Although its first military use in Ypres was 100 years ago, no causal therapy for sulfur mustard (SM) intoxications exists so far. To improve the therapeutic options for the treatment of SM intoxications, we developed a co-culture of keratinocytes (HaCaT cells) and immunocompetent cells (THP-1 cells) to identify potential substances for further research. Here, we report on the influence of necrosulfonamide (NSA) on the course of a SM intoxication in vitro. The cells were challenged with...
1 CitationsSource
#1Thilo SimonsH-Index: 3
#2Dirk Steinritz (LMU: Ludwig Maximilian University of Munich)H-Index: 22
Last. Kai KeheH-Index: 30
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Abstract The chemical warfare agent sulfur mustard (SM) can cause long-term health effects that may occur even years after a single exposure. The underlying pathophysiology is unknown, but epigenetic mechanisms are discussed as feasible explanation. “Epigenetics” depicts regulation of gene function without affecting the DNA sequence itself. DNA-methylation and covalent histone modifications (methylation or acetylation) are regarded as important processes. In the present in vitro study using earl...
3 CitationsSource
#1Dirk Steinritz (LMU: Ludwig Maximilian University of Munich)H-Index: 22
#2Franziska Zehfuß (LMU: Ludwig Maximilian University of Munich)H-Index: 2
Last. Thomas GudermannH-Index: 81
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Abstract The hygroscopic zinc chloride (ZnCl 2 ) is often used to generate smoke screens. Severe adverse pulmonary health effects have been associated with inhalation of ZnCl 2 smokes. The underlying molecular toxicology is not known. Recent studies have shown that the Transient Receptor Potential Channel A1 (TRPA1) is important for sensing toxic chemicals. TRPA1 was shown to be activated by Zn 2+ which was linked to pain and inflammation. In the present study, we investigated whether TRPA1 acti...
6 CitationsSource
#1Simon LangH-Index: 3
#2Tanja Popp (LMU: Ludwig Maximilian University of Munich)H-Index: 15
Last. Dirk Steinritz (LMU: Ludwig Maximilian University of Munich)H-Index: 22
view all 10 authors...
Abstract Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris , which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well...
8 CitationsSource