Zhifeng Xiao
Chinese Academy of Sciences
AnatomyNeural stem cellPathologyCellular differentiationNeurotrophic factorsRegeneration (biology)ChemistryMesenchymal stem cellProgenitor cellImmunologySpinal cord injurySpinal cordLesionStem cellTransplantationTissue engineeringMedicineBiologyScaffoldCell biology
142Publications
49H-index
4,251Citations
Publications 138
Newest
#1Muya Shu (CAS: Chinese Academy of Sciences)H-Index: 2
#2Xiaoyu Xue (CAS: Chinese Academy of Sciences)H-Index: 3
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 17 authors...
Neural stem cells (NSCs) in the spinal cord hold great potential for repair after spinal cord injury (SCI). The ependyma in the central canal (CC) region has been considered as the NSCs source in the spinal cord. However, the ependyma function as NSCs after SCI is still under debate. We used Nestin as a marker to isolate potential NSCs and their immediate progeny, and characterized the cells before and after SCI by single-cell RNA-sequencing (scRNA-seq). We identified two subgroups of NSCs: the ...
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#1Wen Yin (CSU: Central South University)H-Index: 4
#2Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 19 authors...
Scar tissue removal combined with biomaterial implantation is considered an effective measure to repair chronic transected spinal cord injury (SCI). However, whether more scar tissue removal surgeries could affect the treatment effects of biomaterial implantation still needs to be explored. In this study, we performed the first scar tissue removal surgery in the 3rd month and the second in the 6th month after completely removing 1 cm of spinal tissue in canines. We found that Taxol-modified line...
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#1Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
#2Haipeng Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 13 authors...
BACKGROUND Microtubule-stabilizing agents have been demonstrated to modulate axonal sprouting during neuronal disease. One such agent, Epothilone D, has been used to treat spinal cord injury (SCI) by promoting axonal sprouting at the lesion site after SCI. However, the role of Epothilone D in the differentiation of neural stem cells (NSCs) in SCI repair is unknown. In the present study, we mainly explored the effects and mechanisms of Epothilone D on the neuronal differentiation of NSCs and reve...
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#1Xing LiH-Index: 18
#1Li Xing (CSU: Central South University)H-Index: 3
Last. Jianwu DaiH-Index: 69
view all 10 authors...
The limited regrowth of transected axons and insufficient regeneration of lost neurons in adult mammals collectively hinder complete spinal cord injury (SCI) repair. Hence, designing an ideal bio-scaffold which could coordinate the regeneration of axons and neurons in situ might be able to effectively facilitate the reconstruction of neural circuits and the recovery of nerve function after complete SCI. In this study, a sponge-like collagen scaffold with good drug release characteristics and goo...
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#1Lulu ZhangH-Index: 2
#2Caixia FanH-Index: 9
Last. Jianwu DaiH-Index: 69
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#1Xiaoyu Xue (CAS: Chinese Academy of Sciences)H-Index: 3
#2Muya Shu (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 14 authors...
Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury (SCI), Nestin expression is reactivated and Nestin-positive (Nestin+) cells aggregate at the injury site. However, the derivation of Nestin+ cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamo...
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#1Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
#2Caixia Fan (CAS: Chinese Academy of Sciences)H-Index: 9
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 6 authors...
Spinal cord injury (SCI) typically results in long-lasting functional deficits, largely due to primary and secondary white matter damage at the site of injury. The transplantation of neural stem cells (NSCs) has shown promise for re-establishing communications between separated regions of the spinal cord through the insertion of new neurons between the injured axons and target neurons. However, the inhibitory microenvironment that develops after SCI often causes endogenous and transplanted NSCs ...
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#1Haipeng Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
#2Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 11 authors...
Abstract Studying the spatiotemporal dynamic changes of various cells following spinal cord injury (SCI) is of great significance for understanding the pathological processes of SCI. Changes in the characteristics of Sox9-positive cells, which are widely present in the spinal cord, have rarely been studied following SCI. We found that Sox9-positive cells were widely distributed in the central canal and parenchyma of the uninjured adult spinal cord, with the greatest distribution in the central s...
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#1Yi CuiH-Index: 10
#2Yanyun Yin (CAS: Chinese Academy of Sciences)H-Index: 4
Last. Ya Shi (CAS: Chinese Academy of Sciences)H-Index: 4
view all 13 authors...
BACKGROUND Recently, neural stem cell (NSC) therapy has shown promise for the treatment of many neurological diseases. Enhancing the quality of implanted cells and improving therapeutic efficacy are currently research hotspots. It has been reported that collagen sponge material provided sufficient room for cell growth in all directions and promoted the absorption of nutrients and removal of wastes. And also, the Rotary Cell Culture System (RCCS), which mimics the microgravity environment, can be...
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#1Yaming Yang (CAS: Chinese Academy of Sciences)H-Index: 1
#2Yongheng Fan (CAS: Chinese Academy of Sciences)H-Index: 4
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 13 authors...
Complete spinal cord injury (SCI) leads to cell death, interruption of axonal connections and permanent functional impairments. In the development of SCI treatments, cell transplantation combined with biomaterial-growth factor-based therapies have been widely studied. Another avenue worth exploring is the generation of neurons from endogenous neural stem cells (NSCs) or reactive astrocytes activated by SCI. Here, we screened a combination of four small molecules, LDN193189, SB431542, CHIR99021 a...
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