Valentina Doldi
University of Milan
Cell cycle checkpointCancerInternal medicineOncologymicroRNACellGene expressionCytotoxic T cellChemistryApoptosisImmunologySurvivinTumor microenvironmentDU145PaclitaxelMetastasisDiseaseProstate cancerNuclear proteinCancer researchBioinformaticsCell growthStereochemistryMedicineCell cultureBiologyCancer cell
16Publications
8H-index
308Citations
Publications 16
Newest
#1Silvia MartiniH-Index: 3
#2Valentina ZucoH-Index: 25
Last. Nadia ZaffaroniH-Index: 66
view all 10 authors...
Triple-negative breast cancer (TNBC) is an aggressive disease with limited therapeutic options. Here, we pursued a combinatorial therapeutic approach to enhance the activity of selinexor, the first-in-class XPO1 inhibitor, by miR-34a ectopic expression in human TNBC experimental models. Anti-proliferative activity induced by selinexor and miR-34a expression, singly and in combination, was evaluated by MTS assay and cell counting. The effect of treatments on survivin and apoptosis-related protein...
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#1Paolo GandelliniH-Index: 25
Last. Nadia ZaffaroniH-Index: 66
view all 16 authors...
Active surveillance (AS) has evolved as a strategy alternative to radical treatments for very low risk and low-risk prostate cancer (PCa). However, current criteria for selecting AS patients are still suboptimal. Here, we performed an unprecedented analysis of the circulating miRNome to investigate whether specific miRNAs associated with disease reclassification can provide risk refinement to standard clinicopathological features for improving patient selection. The global miRNA expression profi...
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#1Valentina DoldiH-Index: 8
#2Rihan El BezawyH-Index: 3
Last. Nadia ZaffaroniH-Index: 66
view all 3 authors...
Prostate cancer (PCa) is the second most common tumor in men worldwide, and the fifth leading cause of male cancer-related deaths in western countries. PC is a very heterogeneous disease, meaning that optimal clinical management of individual patients is challenging. Depending on disease grade and stage, patients can be followed in active surveillance protocols or undergo surgery, radiotherapy, hormonal therapy, and chemotherapy. Although therapeutic advancements exist in both radiatiotherapy an...
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#1Rihan El BezawyH-Index: 3
#2Martina Tripari (University of Liverpool)H-Index: 1
Last. Nadia ZaffaroniH-Index: 66
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Speckle-type POZ (pox virus and zinc finger protein) protein (SPOP) is the most commonly mutated gene in prostate cancer (PCa). Recent evidence reports a role of SPOP in DNA damage response (DDR), indicating a possible impact of SPOP deregulation on PCa radiosensitivity. This study aimed to define the role of SPOP deregulation (by gene mutation or knockdown) as a radiosensitizing factor in PCa preclinical models. To express WT or mutant (Y87N, K129E and F133V) SPOP, DU145 and PC-3 cells were tra...
1 CitationsSource
#1Federica RotundoH-Index: 2
#2Denis CominettiH-Index: 14
Last. Paolo GandelliniH-Index: 25
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The development of novel therapies or the improvement of currently used approaches to treat prostate cancer (PCa), the most frequently diagnosed male tumor in developed countries, is an urgent need. In this regard, the functional characterization of microRNAs, molecules shown to regulate a number of cancer-related pathways, is instrumental to their possible clinical exploitation. Here, we demonstrate the tumor-suppressive role of the so far uncharacterized miR-1272, which we found to be signific...
1 CitationsSource
#1Paolo GandelliniH-Index: 25
#2Nicola Casiraghi (University of Trento)H-Index: 7
Last. Nadia ZaffaroniH-Index: 66
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Abstract Background Genomic characterization of prostate cancer (PCa) biopsies may improve criteria for the selection of patients suitable for active surveillance (AS). Objective To identify somatic genomic aberrations associated with adverse outcome as AS protocol exclusion indicators. Design, setting and participants Whole-exome sequencing profiles were generated for Gleason score (GS) = 3 + 3 biopsies obtained from 54 PCa patients enrolled in two AS protocols. Patients were selected as repres...
1 CitationsSource
Background Radiotherapy is one of the main treatment options for non-metastatic prostate cancer (PCa). Although treatment technical optimization has greatly improved local tumor control, a considerable fraction of patients still experience relapse due to the development of resistance. Radioresistance is a complex and still poorly understood phenomenon involving the deregulation of a variety of signaling pathways as a consequence of several genetic and epigenetic abnormalities. In this context, c...
28 CitationsSource
#1Valentina ProfumoH-Index: 7
#2Barbara ForteH-Index: 2
Last. Paolo GandelliniH-Index: 25
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Though miR-205 function has been largely characterized, the nature of its host gene, MIR205HG, is still completely unknown. Here, we show that only lowly expressed alternatively spliced MIR205HG transcripts act as de facto pri-miRNAs, through a process that involves Drosha to prevent unfavorable splicing and directly mediate miR-205 excision. Notably, MIR205HG-specific processed transcripts revealed to be functional per se as nuclear long noncoding RNA capable of regulating differentiation of hu...
21 CitationsSource
#1Paolo GandelliniH-Index: 25
#2Valentina DoldiH-Index: 8
Last. Nadia ZaffaroniH-Index: 66
view all 3 authors...
Abstract microRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. Increasing evidence emerging from human tumor preclinical models clearly indicates that specific miRNAs, collectively termed “metastamirs,” play a functional role in different steps of the metastatic cascade, by exerting either pro- or anti-metastatic functions, and behave as signaling mediators to enable tumor cell to colonize a specific organ. miRNAs also actively p...
29 CitationsSource
Metastatic prostate cancer is a lethal disease that remains incurable despite the recent approval of new drugs, thus making the development of alternative treatment approaches urgently needed. A more precise understanding of the molecular mechanisms underlying prostate cancer dissemination could lead to the identification of novel therapeutic targets for the design of efficient anti-metastatic strategies. MicroRNA (miRNAs) are endogenous, small non-coding RNA molecules acting as key regulators o...
17 CitationsSource