Ralph A. Bradshaw
University of California, Irvine
NeuriteInternal medicineEndocrinologyMolecular biologyPeptide sequenceReceptorEnzymeChemistryGrowth factorEpidermal growth factorNerve growth factorChromatographyAmino acidBiochemistrySignal transductionStereochemistryFibroblast growth factorBiologyNeurotrophinCell biology
320Publications
79H-index
13.9kCitations
Publications 310
Newest
#1Katalin F. Medzihradszky (UCSF: University of California, San Francisco)H-Index: 70
#2Zsuzsanna Darula (MTA: Hungarian Academy of Sciences)H-Index: 21
Last. Alma L. Burlingame (UCSF: University of California, San Francisco)H-Index: 120
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Protein sulfonation on serine and threonine residues is described for the first time. This post-translational modification is shown to occur in proteins isolated from organisms representing a broad span of eukaryote evolution, including the invertebrate mollusk Lymnaea stagnalis, the unicellular malaria parasite Plasmodium falciparum, and humans. Detection and structural characterization of this novel post-translational modification was carried out using liquid chromatography coupled to electros...
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#1Roger L. Lundblad (UNC: University of North Carolina at Chapel Hill)H-Index: 24
#2Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
Last. Kenneth G. Mann (UVM: University of Vermont)H-Index: 119
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Thrombin is the product of the hemostatic response essential to the conversion of fibrinogen to fibrin. In addition, it is also responsible for the aggregation of blood platelets in the formation of the platelet plug as well as the activation of factor VIII, factor V, factor XI, factor XIII and protein C. The action of thrombin is not confined to the hemostatic response as it also has a critical function in the wound healing process by stimulating 'mitogenic' events through interaction with cell...
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#1Juan Carlos Jimenez (UCI: University of California, Irvine)H-Index: 11
#2Darren R. TysonH-Index: 24
Last. Gregory R. D. EvansH-Index: 60
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The development of engineered constructs to bridge nerve gaps may hold the key to improved functional outcomes in the repair of injured peripheral nerves. These constructs must be rendered bioactive by providing the growth factors required for successful peripheral nerve regeneration. Previous studies demonstrated that harvested human and rat dermal fibroblasts could be genetically engineered to release nerve growth factor (NGF) both in vitro and in vivo. The use of fibroblasts, however, has the...
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#1Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
#2Elizabeth Yi (UCI: University of California, Irvine)H-Index: 3
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Publisher Summary This chapter elaborates the activity, specificity and structural chemistry of methionyl aminopeptidase type 2. The enzymes best termed methionyl aminopeptidases, but also commonly called methionine aminopeptidases that are active in processing the N-termini of proteins, particularly co-translationally, are metalloenzymes. The two classes of MetAPs share a similar substrate profile but there is growing evidence that there are some more subtle differences in substrate usage. The ...
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#1Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
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#1Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
#2Elizabeth Yi (UCI: University of California, Irvine)H-Index: 3
Last. Kenneth W. Walker (Protein Sciences)H-Index: 2
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Publisher Summary This chapter elaborates the activity, specificity and structural chemistry of methionyl aminopeptidase type 1 (MetAP 1). Two classes of aminopeptidases have been isolated that are involved in the nonprocessive cleavage of the methionine + residue that initiates protein synthesis. Both classes of MetAPs have the same general specificity based on the penultimate residue. MetAPs can be distinguished from a number of other aminopeptidases that catalyze the release of several hydrop...
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#1Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
Methionine, used to initiate protein synthesis in eukaryotes, is largely recovered as a co-translational hydrolysis event catalysed by two related enzymes, methionine aminopeptidase (MetAP)1 and 2. One of these, MetAP2, is covalently modified, and thus catalytically inactivated, by a class of drugs related to the fungal polyene antibiotic, fumagillin. This irreversible inhibition results in cell cycle arrest in endothelial cells (as well as a number of other cells) and prevents vascular growth. ...
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#1Ralph A. BradshawH-Index: 79
#2Edward A. DennisH-Index: 113
Cell Signaling - Yesterday, Today and Tomorrow Initiation: Extracellular and Membrane Events Molecular Recognition Vertical Receptors Horizontal Receptors Membrane Proximal Events Transmission: Effectors and Cytosolic Events Protein Phosphorylation Transmission: Effectors and Cytosolic Events Calcium Mobilization Lipid-Derived Second Messengers Protein Proximity Interactions Cyclic Nucleotides G Proteins Developmental Signaling Nuclear Responses: Gene Expression and Translation Nuclear Receptors...
#1Shivanand P. Lad (UHSA: University of Health Sciences Antigua)H-Index: 43
#2Daniel A. PetersonH-Index: 60
Last. Kenneth E. NeetH-Index: 33
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Abstract A long-standing question in neurotrophin signal transduction is whether heteromeric TrkA-p75NTR complexes possess signaling capabilities that are significantly different from homo-oligomeric TrkA or p75NTR alone. To address this issue, various combinations of transfected PC12 cells expressing a platelet-derived growth factor receptor-TrkA chimera and the p75NTR-selective nerve growth factor mutant (Δ9/13 NGF) were utilized to selectively stimulate TrkA or p75NTR signaling, respectively....
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#1Darren R. Tyson (UCI: University of California, Irvine)H-Index: 24
#2Selena Larkin (UCI: University of California, Irvine)H-Index: 1
Last. Ralph A. Bradshaw (UCI: University of California, Irvine)H-Index: 79
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Abstract PC12 cells have been used as a model system for neuronal differentiation due to their ability to alter their phenotype to a sympathetic neuron-like cell in response to nerve growth factor or fibroblast growth factor. Under some conditions, epidermal growth factor (EGF) can also induce PC12 cells to differentiate. To study signaling from the EGF receptor without the confounding effects of endogenous EGF receptors we generated a chimeric receptor comprised of the ectodomain of platelet-de...
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