José de Jesús Olivares-Trejo
Universidad Autónoma de la Ciudad de México
PromoterGeneColloidal goldGenomeMembrane proteinBacterial outer membraneMolecular biologyPeptide sequenceBacterial growthInclusion bodiesTransferrinHuman pathogenIn silicoPneumococcal infectionsHelicobacter pyloriPathogenEntamoeba histolyticaHemoglobinLactoferrinStreptococcus pneumoniaeHemoglobin bindingRecombinant DNAPlasma protein bindingBacteriaBinding proteinBiochemistryCell growthBiologyMicrobiology
20Publications
13H-index
205Citations
Publications 20
Newest
#2E. M. Gutiérrez-Cárdenas (UAM: Universidad Autónoma Metropolitana)H-Index: 1
Last. O. Hernández-GonzálezH-Index: 1
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Nanotechnology is a frequent treatment for cancer. Nanomaterials are the vehicles which deliver drugs in smaller but equally effective quantities. The aim of this investigation is to synthesize gold nanoparticles, functionalize them for the transportation of cisplatin and release them to the cancer-affected area. They have the same cytotoxicity as conventional treatments but with the smallest effective quantity of cisplatin. We synthesized spherical gold nanoparticles using the Turkevich method....
2 CitationsSource
#1Edgar Augusto Ortiz-Benítez (IPN: Instituto Politécnico Nacional)H-Index: 1
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
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Streptococcus pneumoniae is a causal agent of otitis media, pneumonia, meningitis and severe cases of septicemia. This human pathogen infects elderly people and children with a high mortality rate of approximately one million deaths per year worldwide. Antibiotic-resistance of S. pneumoniae strains is an increasingly serious health problem; therefore, new therapies capable of combating pneumococcal infections are indispensable. The application of gold nanoparticles has emerged as an option in th...
16 CitationsSource
Introduction: Helicobacter pylori is a gram-negative spiral bacterial, has been associated with peptic ulcers, gastritis, duodenitis, and is believed to be the causative agent of gastric cancer. The sources such as human lactoferrin, haem and haemoglobin can support the H. pylori growth. However, still not fully understood how the process of iron acquisition occurs. An in silico analysis has shown that H. pylori genome has a family of three outer membrane protein regulated by iron (FrpB). Two of...
Source
#2Mario A. RodríguezH-Index: 18
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
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Streptococcus pneumoniae is a Gram-positive microorganism that is the cause of bacterial pneumonia, sinusitis and otitis media. This human pathogen also can cause invasive diseases such as meningitis, bacteremia and septicemia. Haemoglobin (Hb) and haem can support the growth and viability of S. pneumoniae as sole iron sources. Unfortunately, the acquisition mechanism of Hb and haem in this bacterium has been poorly studied. Previously we identified two proteins of 37 and 22 kDa as putative Hb- ...
5 CitationsSource
Antimicrobial resistance is considered a public health problem around the world. Every day new resistance mechanisms emerge and spread globally. Therefore, is imperative to improve the treatment schemes that have been developed to cure infections caused by human pathogens for instance, Streptococcus pneumoniae. Here, we are proposing a strategy that consists on combine the drug with iron oxide nanoparticles (FeNPs). We synthetized FeNPs by chemical technique and they were functionalized in order...
12 CitationsSource
#1Edgar Augusto Ortiz-Benitez (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 1
#2Mariana Carrillo-Morales (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 2
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
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Streptococcus pneumoniae is a human pathogen whose principal virulence factor is its capsule. This structure allows the bacterium to evade the human immune system. Treatment of infections caused by this bacterium is based on antibiotics; however, the emergence of antibiotic-resistant strains makes this task increasingly difficult. Therefore, it is necessary to investigate new therapies, such as those based on gold nanoparticles, for which unfortunately the mechanisms involved have not yet been i...
10 CitationsSource
Staphylococcus aureus is an opportunistic pathogen that colonizes human hosts and causes a wide variety of diseases. Two interacting regulatory systems called agr (accessory gene regulator) and sar (staphylococcal accessory regulator) are involved in the regulation of virulence factors. The aim of this study was to evaluate the effect of vancomycin on hld and spa gene expression during the exponential and post-exponential growth phases in multidrug resistant (MDR) S. aureus. Methods. Antibiotic ...
10 CitationsSource
#1Zelene Edith Vázquez-Zamorano (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 1
#2Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
view all 6 authors...
Streptococcus pneumoniae is a gram positive encapsulated bacterium responsible of septicaemia and upper respiratory infections in children. This pathogen requires iron to survive in the host, which it can obtain of haemoglobin (Hb) or haem. Only two Hb-binding membrane proteins have been identified up to now. However it is unknown whether this pathogen secretes proteins in order to scavenge iron from the Hb or haem. Therefore, in order to explore these possibilities, cellular growth of S. pneumo...
6 CitationsSource
#1Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
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Helicobacter pylori is a bacterium that can use multiple iron sources. However, it is unknown whether this bacterium secretes molecules such as siderophores or haemophores to scavenge iron. Here, we report the first secreted iron-binding protein of H. pylori, which we purified by haem-affinity chromatography. Mass spectrometry analysis revealed its identity as chaperonin (HpGroEL). When we compared HpGroEL with EcGroEL from Escherichia coli, they were homologous, showing 60% similarity. Addition...
20 CitationsSource
#1María Elena Romero-Espejel (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
#2Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
view all 3 authors...
Streptococcus pneumoniae, a human pathogen bacterium, can support its growth using haemoglobin (Hb) and haem as sole iron sources, but not when holo-transferrin or holo-lactoferrin is supplied. For this reason, it is easy to think that the principal iron sources for this pathogen inside humans are Hb and haem. Unfortunately, the mechanism has been poorly studied. The findings presented in this study are the first efforts that attempted to explain the mechanism involved in iron acquisition of thi...
24 CitationsSource