Gokhan Yahioglu
Imperial College London
Fluorescence anisotropyAnalytical chemistryCancerBiophysicsViscosityMicroviscosityPathologyAntibodyNanotechnologyLight-emitting diodeChemistryMaterials scienceConjugateIn vitroMUC1PorphyrinDrugPhotodynamic therapyCancer researchFluorescence-lifetime imaging microscopyMedicinePhotochemistryFluorescence
65Publications
24H-index
2,097Citations
Publications 62
Newest
#1Yilei WuH-Index: 27
#2Martin ŠteflH-Index: 10
Last. Marina K. KuimovaH-Index: 38
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Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy and imaging (FLIM) of a meso-substituted BODIPY molecular rotor localised in the leaflet of model membranes of various lipid compositions. We prepare large and giant unilamella...
#1Mohammed A. ButtH-Index: 9
#2Hayley PyeH-Index: 9
Last. Laurence LovatH-Index: 36
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Introduction Most patients with oesophageal adenocarcinoma (OA) are unfit for curative treatment but are fit for photodynamic therapy (PDT). PDT has been limited by poor tumour selectivity, poor tissue penetration of light and protracted side effects. These restrictions may be overcome with targeted PDT. We have previously demonstrated aberrantly glycosylated MUC1 (AG-MUC1), bound by humanised anti-human milk-fat globule-1 (Hu-HMFG1) antibody is significantly expressed in Barrett9s epithelium (B...
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#1Mohammed A. ButtH-Index: 9
#2Ioanna StamatiH-Index: 5
Last. Laurence LovatH-Index: 36
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Introduction Targeted photodynamic therapy (PDT) has the potential to overcome current limitations of PDT agents by offering specific tumour kill with reduced side effects. We have previously shown expression of AG-MUC1, an epithelial cancer target bound by the antibody HuHMFG1, in patients with Barrett9s with dysplasia, oesophageal adenocarcinoma (OA) and on OE19, an OA cell line. We have recently developed and characterised photoimmunoconjugates (PIC) of Hu-HMFG1 with the photosensitiser PS1 t...
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#1Mohammed A. ButtH-Index: 9
#2Ioanna StamatiH-Index: 5
Last. Laurence LovatH-Index: 36
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#1Mohammed A. ButtH-Index: 9
#2Rehan HaidryH-Index: 16
Last. Laurence LovatH-Index: 36
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Introduction There is increasing attention on the integration of targeted agents for oesophageal adenocarcinoma (OA) therapy. The most notable example of success of oesophagogastric targeted therapy was the addition of a HER2 targeting agent in the Phase III ToGA study. However, there is limited data on the expression of HER2 in the progression from Barrett9s (BE) to OA. This study aimed to clarify expression in this sequence, and to show binding of C6.5, a single chain HER2 targeting human anti...
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#1Mohammed A. ButtH-Index: 9
#2Rehan HaidryH-Index: 16
Last. Laurence LovatH-Index: 36
view all 11 authors...
#1Mohammed A. ButtH-Index: 9
#2Hayley PyeH-Index: 9
Last. Laurence LovatH-Index: 36
view all 9 authors...
#1Mahendra Deonarain (Imperial College London)H-Index: 19
#2Ioanna Stamati (Imperial College London)H-Index: 5
Last. Gokhan YahiogluH-Index: 24
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Photodynamic therapy (PDT) is a clinically approved treatment modality for cancer and superficial lesions. It harnesses the photochemical reaction between the lightsensitizing drug and oxygen to generate cytotoxic reactive oxygen species which destroy cells. Although with many benefits, its complexity, lack of potency and side effects have led to poor general usage. However, the research area is very active with an increasing understanding of PDT-related cell biology, photophysics and significan...
1 CitationsSource
#1Klaus Suhling ('KCL': King's College London)H-Index: 41
#2James A. Levitt ('KCL': King's College London)H-Index: 21
Last. Gokhan YahiogluH-Index: 24
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Diffusion is often an important rate-determining step in chemical reactions or biological processes and plays a role in a wide range of intracellular events. Viscosity is one of the key parameters affecting the diffusion of molecules and proteins, and changes in viscosity have been linked to disease and malfunction at the cellular level.1-3 While methods to measure the bulk viscosity are well developed, imaging microviscosity remains a challenge. Viscosity maps of microscopic objects, such as si...
10 CitationsSource
#1Klaus Suhling ('KCL': King's College London)H-Index: 41
#2Nicholas I. Cade ('KCL': King's College London)H-Index: 18
Last. David Richards ('KCL': King's College London)H-Index: 26
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Fluorescence lifetime imaging (FLIM) is a key optical technique for imaging the photophysical environment of fluorescent probes in vivo. In addition, it also provides information in fluorescence enhancement studies that intensity imaging alone could not provide. We review the principles and recent advances in the application of the techniques, instrumentation and molecular probe development.
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