Makoto Muroi
Tohoku University
ProteomeMolecular biologyEndoplasmic reticulumSmall moleculeEnzymeChemistryIn vitroCombinatorial chemistryMechanism of actionHeLaTarget proteinProteomic ProfilingProgrammed cell deathProteomicsSecretionBiochemistryStereochemistryIntracellularCell cultureBiologyCell biologyCancer cell
75Publications
19H-index
1,247Citations
Publications 74
Newest
#1Thanet Sophonnithiprasert (Rangsit University)H-Index: 4
#2Ornjira Aruksakunwong (Rangsit University)H-Index: 12
Last. Ramida Watanapokasin (SWU: Srinakharinwirot University)H-Index: 14
view all 8 authors...
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#1Thanet Sophonnithiprasert (Rangsit University)H-Index: 4
#2Ornjira Aruksakunwong (Rangsit University)H-Index: 12
Last. Ramida Watanapokasin (SWU: Srinakharinwirot University)H-Index: 14
view all 8 authors...
Endoplasmic reticulum stress is one of the pathways involved in cell cytotoxicity. In this study, goniothalamin, one of styryllactone compounds found in plant Goniothalamus spp., was observed to trigger ER stress in HeLa cell line. In addition, we demonstrated that peroxisomal multifunctional enzyme type2 (MFE2) was a specific goniothalamin-binding protein using an in vitro goniothalamin-linked bead pull-down assay. Since MFE2 has been reported to be an important mediator enzyme for peroxisomal ...
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#1Tatsuro Kawamura (MPG: Max Planck Society)H-Index: 5
#2Yushi FutamuraH-Index: 16
Last. Hiroyuki OsadaH-Index: 79
view all 13 authors...
Discovery of small-molecule inducers of unique phenotypic changes combined with subsequent target identification often provides new insights into cellular functions. Here, we applied integrated pro...
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#1Hiroaki Ikeda (Kyoto University)H-Index: 1
#2Makoto MuroiH-Index: 19
Last. Masaya Imoto (Keio: Keio University)H-Index: 36
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The Wnt signaling pathway regulates diverse cellular processes. β-catenin is one of the major components of this pathway, in which it plays a main role. Although it has been established that β-cate...
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#1Kaori Sako (Kindai University)H-Index: 1
#1Kaori Sako (Kindai University)H-Index: 7
Last. Motoaki SekiH-Index: 106
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Chemical priming is an attractive and promising approach to improve abiotic stress tolerance in a broad variety of plant species. We screened the RIKEN Natural Products Depository (NPDepo) chemical library and identified a novel compound, FSL0260, enhancing salinity-stress tolerance in Arabidopsis thaliana and rice. Through transcriptome analysis using A. thaliana seedlings, treatment of FSL0260 elevated an alternative respiration pathway in mitochondria that modulates accumulation of reactive o...
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#1Ikuko NagasawaH-Index: 1
#2Makoto MuroiH-Index: 19
Last. Hiroyuki OsadaH-Index: 79
view all 7 authors...
Summary The cellular thermal shift assay (CETSA) has recently been devised as a label-free method for target validation of small compounds and monitoring the thermal stabilization or destabilization of proteins due to binding with the compound. Herein, we developed a modified method by combining the CETSA and proteomics analysis based on 2D gel electrophoresis, namely 2DE-CETSA, to identify the thermal stability-shifted proteins by binding with a new compound. We applied the 2DE-CETSA for analys...
5 CitationsSource
#1Kruthi SuvarnaH-Index: 2
#2Kaori HondaH-Index: 11
Last. Nobumoto WatanabeH-Index: 26
view all 6 authors...
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was to study the effect of the compound on ATPase activity in vitro of purified VCP protein. ATPases are enzymes that hydrolyze ATP in a reaction resulting the release of an inorganic phosphate. This reaction can be measured using several methods, such as ...
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#1Kruthi SuvarnaH-Index: 2
#2Kaori HondaH-Index: 11
Last. Hiroyuki OsadaH-Index: 79
view all 6 authors...
Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-target identification. Although there are several other methods used for drug-target identification, such as biochemical fractionation or radioactive ligand binding assay, affinity purification is widely u...
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#1Jing Li (ZJU: Zhejiang University)H-Index: 1
#1Jing Li (ZJU: Zhejiang University)H-Index: 35
Last. Jianhua Qi (ZJU: Zhejiang University)H-Index: 12
view all 8 authors...
: Cucurbitacin B (CuB) isolated from Cucumis melo by using a PC12 cell bioassay system exhibited significant nerve growth factor (NGF)-mimic or NGF-enhancing activity in PC12 and primary neuron cells. It was also demonstrated pro-neurogenesis effects in ICR and APP/PS1 mice and improved memory deficit of APP/PS1 mice. Its possible mechanism includes significant induction of the phosphorylation of glucocorticoid receptor (GR), protein kinase C (PKC), phospholipase C (PLC) and inhibition of cofili...
6 CitationsSource
#1Amit SubediH-Index: 8
#2Makoto MuroiH-Index: 19
Last. Hiroyuki OsadaH-Index: 79
view all 9 authors...
: Differences in the metabolism of cancer cells or cancer stem cells (CSCs) as compared to normal cells have provided avenues to safely target cancers. To discover metabolic inhibitors of CSCs, we performed alkaline phosphatase- and tumoursphere-based drug screening using induced cancer stem cell-like cells. From the screening of a RIKEN NPDepo chemical library, we discovered NPD2381 as a novel and selective cancer-stemness inhibitor that targets mitochondrial metabolism. Using our ChemProteoBas...
2 CitationsSource