Paola Iudicone
Molecular biologyCytotoxic T cellVirologyMesenchymal stem cellImmunologyPlatelet lysateInterleukin 2HBsAgThymoglobulinImmunotherapyCytokine-induced killer cellBone marrowHepatitis B virusLymphokine-activated killer cellMedicineInterleukinCell cultureBiologyCell biologyInterleukin 15
17Publications
10H-index
317Citations
Publications 17
Newest
#1Paola IudiconeH-Index: 10
#2Daniela FioravantiH-Index: 7
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 8 authors...
Abstract CIK cells are a subset of effector lymphocytes endowed with a non-MHC restricted anti-tumor activity making them an appealing and promising cell population for adoptive immunotherapy. CIK are usually generated ex-vivo by initial priming with Interferon-γ (IFN-γ) and monoclonal antibody against CD3 (anti-CD3), followed by culture in medium containing Interleukin-2 (IL-2). Interleukin-15 (IL-15) shares with IL-2 similar biological functions and recently it has been reported to induce CIK ...
5 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 7 authors...
Background Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biological features, although they may show some differences on cumulative yield, proliferative ability and differentiation potential. The standardization of MSCs growth and their funct...
21 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca PierelliH-Index: 34
view all 9 authors...
Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in ce...
17 CitationsSource
#1Maria Beatrice Rondinelli (RMIT: RMIT University)H-Index: 2
Last. Luca PierelliH-Index: 34
view all 11 authors...
Background: Oral iron support in patients (pts) undergoing preoperative autologous blood donation (PABD) programs has been suggested by different authors to improve red blood cell (RBC) production and limit iron depletion. Oral iron therapy, however, is frequently associated with side effects and poor pts compliance. The aim of this study was to evaluate the effectiveness of low doses of ferrous bisglycinate chelate (Tecnofer, Baldacci) a new oral iron preparation characterized by high gastroint...
1 CitationsSource
#1Camilla Siciliano (IIT: Istituto Italiano di Tecnologia)H-Index: 10
#2Isotta ChimentiH-Index: 22
Last. Elena De FalcoH-Index: 19
view all 11 authors...
Human adipose tissue-derived mesenchymal stem cells (ADMSCs) are considered eligible candidates for cardiovascular stem cell therapy applications due to their cardiac transdifferentiation potential and immunotolerance. Over the years, the in vitro culture of ADMSCs by platelet lysate (PL), a hemoderivate containing numerous growth factors and cytokines derived from platelet pools, has allowed achieving a safe and reproducible methodology to obtain high cell yield prior to clinical administration...
10 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Andrea Mariotti (CUA: The Catholic University of America)H-Index: 14
Last. Giuseppina Bonanno (CUA: The Catholic University of America)H-Index: 33
view all 8 authors...
BACKGROUND Mesenchymal stromal cells (MSCs) isolated from human umbilical cord tissue (UCT) can be considered the perfect candidates for cell-based therapies and regenerative medicine. UCT-derived MSCs can be cryogenically stored in cell banks and expanded as needed for therapeutic uses. STUDY DESIGN AND METHODS We developed a new method for UCT-MSC isolation, cryopreservation, and expansion, following all criteria required by a stem cell bank. UCT-MSCs were isolated either by manual dissociatio...
8 CitationsSource
#2Paola IudiconeH-Index: 10
Last. Luca PierelliH-Index: 34
view all 3 authors...
SUMMARY The storage of hemopoietic progenitor cells (HPC) is a current practice to allow autologous transplantation or, in very rare cases, to collect enough HPC from donors with documented suboptimal characteristics as referred to a given recipient (e.g. high weight disparity) or not endowed with proper vascular accesses (i.e. in case of peripheral HPC collection). In the autologous setting, HPC storage may last several years at very low temperature in tank containing liquid nitrogen, albeit mo...
Source
#1Paola IudiconeH-Index: 10
#2Daniela FioravantiH-Index: 7
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 7 authors...
Source
#1Paola IudiconeH-Index: 10
#2Daniela FioravantiH-Index: 7
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 9 authors...
Background Supplements to support clinical-grade cultures of mesenchymal stem cells (MSC) are required to promote growth and expansion of these cells. Platelet lysate (PL) is a human blood component which may replace animal serum in MSC cultures being rich in various growth factors. Here, we describe a plasma poor pathogen-free platelet lysate obtained by pooling 12 platelet (PLT) units, to produce a standardized and safe supplement for clinical-grade expansion of MSC.
54 CitationsSource
#1Marco SpolitiH-Index: 6
#2Paola IudiconeH-Index: 10
Last. Luca PierelliH-Index: 34
view all 6 authors...
Summary Articular cartilage injuries of the knee are difficult to treat due to the poor healing ability of cartilage and conventional treatment methods often give unsatisfactory results. Mesenchymal Stem Cells (MSCs) have generated interest as an alternative source of cells for cartilage tissue engineering due to their chondrogenic potential and their easy isolation from bone marrow. It has been reported that the use of scaffold in cartilage engineering acts as a support for cell adhesion, keepi...
11 Citations
12
Close Researchers