Jocelyne DiRuggiero
Johns Hopkins University
GeneEcosystemDNA repairGenomeMicrobiomeAridMetagenomicsTranscriptomePeptide sequenceHabitatEcologyExtremophileOxidative stressPyrococcus furiosusHalobacterium salinarumCyanobacteriaGeneticsArchaeaDNAHyperthermophileBiochemistryComputational biologyBiologyMicrobiologyExtreme environment
95Publications
31H-index
3,470Citations
Publications 82
Newest
#1B. Murray (Johns Hopkins University)
#2Micah Dailey (Johns Hopkins University)H-Index: 2
Last. Jocelyne DiRuggiero (Johns Hopkins University)H-Index: 31
view all 4 authors...
Cyanobacteria are essential to microbial communities inhabiting translucent rocks in hyper-arid deserts. Metagenomic studies revealed unique adaptations of these cyanobacteria, but validation of the corresponding metabolic pathways remained challenging without access to isolates. Here, we present high-quality metagenome-assembled genomes for cyanobacteria, and their heterotrophic companions, isolated from endolithic substrates.
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#1Emine Ertekin (Johns Hopkins University)H-Index: 2
#2Victoria Meslier (Johns Hopkins University)H-Index: 3
Last. Jocelyne DiRuggiero (Johns Hopkins University)H-Index: 31
view all 5 authors...
Endolithic (rock-dwelling) microbial communities are ubiquitous in hyper-arid deserts around the world and the last resort for life under extreme aridity. These communities are excellent models to explore biotic and abiotic drivers of diversity because they are of low complexity. Using high-throughput amplicon and metagenome sequencing, combined with X-ray computed tomography, we investigated how water availability and substrate architecture modulated the taxonomic and functional composition of ...
3 CitationsSource
#1Gherman Uritskiy (Johns Hopkins University)H-Index: 6
#2Michael J. Tisza (Johns Hopkins University)H-Index: 7
Last. Jocelyne DiRuggiero (Johns Hopkins University)H-Index: 31
view all 6 authors...
Microbial communities play essential roles in the biosphere and understanding the mechanisms underlying their functional adaptations to environmental conditions is critical for predicting their behavior. This aspect of microbiome function has not been well characterized in natural high-salt environments. To address this knowledge gap, and to build a general framework relating the genomic and transcriptomic components in a microbiome, we performed a meta-omic survey of extremophile communities in...
8 CitationsSource
#1B. Murray (Johns Hopkins University)
#2Micah DaileyH-Index: 2
Last. Jocelyne DiRuggieroH-Index: 31
view all 4 authors...
Cyanobacteria are essential to microbial communities inhabiting translucent rocks in hyper-arid deserts. Metagenomic studies revealed unique adaptations of these cyanobacteria but validation of the corresponding metabolic pathways remained challenging without access to isolates. Here we present high-quality metagenome assembled genomes for cyanobacteria, and their heterotrophic companions, isolated from endolithic substrates.
Source
#1María Cristina Casero (CSIC: Spanish National Research Council)H-Index: 3
#2Victoria Meslier (Johns Hopkins University)H-Index: 3
Last. Jacek Wierzchos (CSIC: Spanish National Research Council)H-Index: 43
view all 8 authors...
Abstract. Endolithic microhabitats have been described as the last refuge for life in arid and hyper-arid deserts where life has to deal with harsh environmental conditions. A number of rock substrates from the hyper-arid Atacama Desert, colonized by endolithic microbial communities such as halite, gypsum crusts, gypcrete, calcite, granite and ignimbrite, have been characterized and compared using different approaches. In this work, three different endolithic microhabitats are described, each on...
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Abstract null null Mars was capable of sustaining life in the Noachian and Hesperian periods (>3 billion years ago). But habitability progressively deteriorated thereafter, and a lasting trend of climate change has made the surface of the planet uninhabitable at present times. One of the outstanding enigmas in Mars exploration is when was the last time that the surface of the planet could have sustained life. We address this question through comparisons with one of the driest regions on Earth, t...
2 CitationsSource
#1Wei Huang (UCR: University of California, Riverside)H-Index: 13
#1Wei Huang (UCR: University of California, Riverside)
Last. David Kisailus (UCR: University of California, Riverside)H-Index: 32
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In response to Wierzchos et al. (1) regarding the mechanism of water extraction from gypsum rock by desert colonizing microorganisms (2), we provide details that refute their incorrect assessments. We carefully selected areas without microorganism colonies that only contained gypsum, as confirmed by X-ray diffraction (XRD), for our culture experiments. Raman is useful for localized analysis of phase on surfaces, but here, it is complementary to XRD, which provides sample-wide detection (3, 4). F...
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#1Gherman UritskiyH-Index: 6
#2Adam MunnH-Index: 3
Last. Jocelyne DiRuggieroH-Index: 31
view all 9 authors...
Spatial heterogeneity in microbial communities is observed in all natural ecosystems and can stem from both adaptations to local environmental conditions as well as stochastic processes. Extremophile microbial communities inhabiting evaporitic halite nodules (salt rocks) in the Atacama Desert, Chile, are a good model ecosystem for investigating factors leading to microbiome heterogeneity, due to their diverse taxonomic composition and the spatial segregation of individual nodules. We investigate...
1 CitationsSource
#1Diego R. GelsingerH-Index: 7
#2Rahul ReddyH-Index: 2
Last. Jocelyne DiRuggieroH-Index: 31
view all 5 authors...
ABSTRACT Haloarchaea are highly resistant to oxidative stress, however, a comprehensive understanding of the processes regulating this remarkable response is lacking. Oxidative stress-responsive small non-coding RNAs (sRNAs) have been reported in the model archaeon, Haloferax volcanii, but targets and mechanisms have not been elucidated. Using a combination of high throughput and reverse molecular genetic approaches, we elucidated the functional role of the most up-regulated intergenic sRNA duri...
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#1Diego R. Gelsinger (Johns Hopkins University)H-Index: 7
#2Emma Dallon (Johns Hopkins University)H-Index: 1
Last. Jocelyne DiRuggiero (Johns Hopkins University)H-Index: 31
view all 6 authors...
High-throughput methods, such as ribosome profiling, have revealed the complexity of translation regulation in Bacteria and Eukarya with large-scale effects on cellular functions. In contrast, the translational landscape in Archaea remains mostly unexplored. Here, we developed ribosome profiling in a model archaeon, Haloferax volcanii, elucidating, for the first time, the translational landscape of a representative of the third domain of life. We determined the ribosome footprint of H. volcanii ...
17 CitationsSource