Salvatore Sotgia
University of Sassari
AnalyteDetection limitInternal medicineEndocrinologyChemistryOxidative stressCOPDInflammationDerivatizationHomocysteineArginineCysteineAsymmetric dimethylarginineThiolCapillary electrophoresisElectrophoresisChromatographyGlutathioneBiochemistryMedicineBiology
144Publications
25H-index
1,900Citations
Publications 145
Newest
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 4 authors...
Malondialdehyde (MDA) determination is the most widely used method for monitoring lipid peroxidation. Here, we describe an easy field-amplified sample injection (FASI) CE method with UV detection for the detection of free plasma MDA. MDA was detected within 8 min by using 200 mmol/L Tris phosphate pH 5.0 as running buffer. Plasma samples treated with ACN for protein elimination were directly injected on capillary without complex cleanup and/or sample derivatization procedures. Using electrokinet...
19 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 6 authors...
Abstract A field amplified sample injection (FASI) capillary electrophoresis method with UV detection was developed for the separation and detection of carnosine-related peptides carnosine (Car), anserine (Ans) and homocarnosine (Hcar). The imidazole dipeptides were baseline-separated within 10 min by using 50 mmol/L Tris phosphate pH 2.2 as running buffer. The samples were diluted in water and directly injected on the capillary without complex cleanup and/or sample derivatization procedures. Us...
14 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore SotgiaH-Index: 25
Last. Ciriaco CarruH-Index: 38
view all 8 authors...
BACKGROUND: The aim of this work has been to study the association between plasma asymmetric dimethylarginine (ADMA) concentrations and carotid stenosis in a group of 64 patients undergoing carotid endarterectomy (CEA). METHODS: Arginine, ADMA and symmetric dimethylarginine (SDMA) were measured using capillary electrophoresis with UV detection. An evaluation of plasma concentrations of total cysteine (tCys) and total homocysteine (tHcy) was also performed. RESULTS: Pearson's analysis show a posi...
24 CitationsSource
#1Ciriaco CarruH-Index: 38
#2Valeria PasciuH-Index: 14
Last. Gianfranco PintusH-Index: 32
view all 10 authors...
Antioxidants exert contrasting effect on low density lipoprotein (LDL) oxidation catalysed by metals, acting as pro-oxidants under select in vitro conditions. Through our study on the effect of coffee on LDL oxidation, we identified the parameters governing this phenomenon, contributing to the comprehension of its mechanism and discovering significant implications for correct alimentary recommendations. By measuring conjugated diene formation, we have analysed the quantitative and qualitative ef...
9 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 6 authors...
Here, we describe an easy field-amplified sample injection capillary electrophoresis method with UV detection for the separation and detection of free plasma arginine and dimethylated arginines. The analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. The plasma samples were treated with acetonitrile/ammonia for protein elimination, the supernatants were dried, re-swollen in water and directly injected in the capillary without complex cleanup...
28 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 4 authors...
The allantoin/uric acid (All/UA) ratio and malondialdehyde (MDA) plasma levels have been proposed as important markers for monitoring oxidation triggered by the action of free radicals (FR). Here, we describe an easy field amplified sample injection capillary electrophoresis method with UV detection for the separation and quantification of All, UA, and free MDA in human plasma. The plasma samples were simply filtered through centrifugation membrane tubes for protein elimination and directly inje...
16 CitationsSource
#1Gian Luca Erre (University of Sassari)H-Index: 17
#2Pietro Sanna (University of Sassari)H-Index: 1
Last. Giuseppe Passiu (University of Sassari)H-Index: 19
view all 9 authors...
Conclusive data about the prevalence of endothelial dysfunction and atherosclerotic process in ankylosing spondylitis (AS) patients with respect to the general population are lacking. Elevated plasma levels of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, have been reported in clinical conditions associated with endothelial dysfunction and atherosclerotic disease. We performed a cross-sectional study to evaluate plasma ADMA levels and atherosclerotic disea...
36 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 11 authors...
Alterations in global DNA methylation are implicated in several pathobiological processes. The tissues stored as paraffin blocks represent an important source of DNA for retrospective genetic and epigenetic analysis on a large scale. Therefore, we developed the first capillary electrophoresis method able to measure global methylation in formalin-fixed, paraffin-embedded (FFPE) DNA extracts. A field-amplified sample injection capillary electrophoresis method with UV detection for the separation a...
16 CitationsSource
#2A MuraH-Index: 6
Last. Paolo Cossu-RoccaH-Index: 16
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Colorectal cancer is ranked the third most common cancer worldwide in terms of incidence and the second in terms of mortality. Recent advances in therapeutic approaches to colorectal cancer have identified a potential role of antiepidermal growth factor receptor (EGFR) targeted therapies as adjuvant treatment in advanced disease. New evidences showed that patients harboring KRAS mutations on codons 12 and 13 are not responsive to anti-EGFR monoclonal antibodies. Therefore, new mutational screeni...
7 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 7 authors...
We describe a new CE method with UV-detection for the quantification of histidine (His) and its methylated forms 1-methylhistidine and 3-methylhistidine, both in plasma and urine. Analytes were basically resolved using a 60 mmol/L Tris-phosphate run buffer pH 2.2 in less than 12 min. The use of a mixture of ACN/ammonia (80:20) for protein precipitation allows the quantitative recovery of all His from plasma. The optimization of the sample volume injection permits to reach an LOD of 20 nmol/L, th...
9 CitationsSource