Salvatore Sotgia
University of Sassari
AnalyteDetection limitInternal medicineEndocrinologyChemistryOxidative stressCOPDInflammationDerivatizationHomocysteineArginineCysteineAsymmetric dimethylarginineThiolCapillary electrophoresisElectrophoresisChromatographyGlutathioneBiochemistryMedicineBiology
144Publications
25H-index
1,900Citations
Publications 145
Newest
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Angelo Zinellu (University of Sassari)H-Index: 31
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 6 authors...
A new hydrophilic interaction ultra-performance LC method was established for the whole blood measurement of L-ergothioneine. Chromatographic separation was achieved in a fairly short time, less than 4 min, on a 100 × 2.1 mm Acquity UPLC BEH HILIC 1.7 μm column with a mobile phase consisting of a mixture of 100 mmol/L ammonium acetate/ACN/water (5:85:10, v/v/v) that flowed isocratically at 0.250 mL/min. The LOD and the limit of quantification were 3.85 and 11.67 μmol/L, respectively. The method ...
14 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 4 authors...
We report a capillary electrophoresis method by field-amplified sample injection (FASI) for the separation and detection of free plasma arginine and dimethylated arginines. Protein elimination from plasma was performed by acetonitrile/ammonia precipitation. Supernatants were dried, reswollen in water, and directly injected on capillary without complex cleanup by solid-phase extraction and/or tedious sample derivatization procedures. Analytes were baseline-separated within 22 min by using 50 mmol...
3 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 4 authors...
: Neurotransmitter amino acids like Alanine (Ala), Glutamic acid (Glu), Aspartic acid (Asp), Serine (Ser), Taurine (Tau), and Glycine (Gly) are widely distributed in biological fluids and tissues and they have important physiological functions, so that the evaluation of their levels in organisms plays an essential role in physiology. We here report a new capillary electrophoresis (CE) method with laser-induced fluorescence detection by which analytes are resolved and detected in less than 12 min...
8 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 7 authors...
Abstract The dyslipidemia control through lipid lowering therapy is one of the targets for the treatment of CKD. By this pilot study we aimed to evaluate the effect of hypolipidemic drugs on the levels of low molecular weight (LMW) thiols bound to LDL in nephropatic patients. We enrolled thirty CKD randomized to receive three different hypolipidemic regimens: simvastatin alone (40 mg/day) or ezetimibe/simvastatin combined therapy (10/20 or 10/40 mg/day). LMW thiols in their reduced and total for...
10 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 6 authors...
Plasma kynurenine (Kyn)/tryptophan ratio has been proposed as a useful marker for the monitoring activation of the cellular immune system. Here, we describe an easy capillary electrophoresis method with UV detection for the separation and detection of Kyn and tryptophan in human plasma using methltryptophan as internal standard. The plasma samples were simply treated with acentonitrile for the elimination of proteins, the supernatant was evaporated, and the dried sample was resuspended with wate...
16 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 4 authors...
We describe the first capillary electrophoresis UV detection method to measure serotonin in human whole blood (WB). Procedural parameters such as concentration and pH of run buffer and injection mode were investigated. The reverse injection allows to decrease the analysis time by injecting samples at the outlet end of the silica capillary close to the detection window, so reducing the migration distance. Thus, when a capillary with an effective length of 10 cm and a 400 mmol/L Tris phosphate as ...
17 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 7 authors...
Abstract Objectives We have investigated on the levels of homocysteine linked to LDL in acute myocardial infarction patients. Design and methods We used capillary electrophoresis to measure LDL-bound thiols in 16 AMI individuals and 32 healthy volunteers. Results We found a significant increase of apo-B homocysteine and cysteine levels in LDL fraction of AMI subjects. Conclusions Our findings suggest that also LDL S-homocysteinylation may be considered among the markers for the CVD risk evaluati...
8 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 6 authors...
Lipid-lowering therapy has been reported to reduce several oxidative stress (OS) markers in hypercholesterolemia. Since OS is frequently associated with renal dysfunction, we aimed to investigate the effect of hypolipidemic drugs on oxidative stress and plasma taurine (Tau), a sulfur amino acid with a marked antioxidant effect, in chronic kidney disease (CKD). We enrolled 30 CKD randomized to receive three different hypolipidemic regimens for 12 months: simvastatin alone (40 mg/day) or ezetimibe...
17 CitationsSource
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Ciriaco Carru (University of Sassari)H-Index: 38
Last. Angelo Zinellu (University of Sassari)H-Index: 31
view all 5 authors...
Modifications of low-density lipoprotein-cholesterol particles (LDLs) are among the principal mechanisms that can play a pivotal role in the initiation and progression of atherosclerosis. It is well known, in fact, that once modified, LDLs increase their atherogenicity, resulting in enhanced uptake by macrophage scavenger receptors that in the long run, leads to the formation of lipid-laden macrophages, or “foam cells,” which are the hallmark of atherosclerotic lesions. Among the atherogenic mod...
2 CitationsSource
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Angelo Zinellu (University of Sassari)H-Index: 31
Last. Ciriaco Carru (University of Sassari)H-Index: 38
view all 5 authors...
An unusual reaction involving ninhydrin and aminothiols was exploited to set an indirect method for the chiral recognition of stereoisomers of penicillamine. Separation of diastereoisomers was achieved on a C18 column in isocratic mode by using a mixture of propionic acid (pH 3.0)/acetonitrile/water (10:10:80, v/v/v) as a mobile phase. Diastereoisomers were detected by a fluorescence detector in fairly short times (about 7 min) and with a good resolution. The lowest detectable amount of toxic is...
5 CitationsSource